- Research article
- Open Access
Investigation of the pathophysiology of cardiopulmonary bypass using rodent extracorporeal life support model
BMC Cardiovascular Disorders volume 17, Article number: 123 (2017)
Extracorporeal life support (ECLS) systems are life-saving devices used for treating patients with severe cardiopulmonary failure. In this study, we implemented a rat model of ECLS without the administration of inotropes or vasopressors.
The rats underwent 5 min of untreated asphyxial cardiac arrest and were resuscitated by ECLS for 30 min. The right external jugular vein and right femoral artery were separately cannulated to the ECLS outflow and inflow, respectively. Thereafter, ECLS was terminated, wounds were closed, and mechanical ventilation was provided for another 90 min. Subsequently, blood gas and hemodynamic analyses were performed. The plasma levels of C-reactive protein (CRP), interleukin (IL)-6, IL-10, and tumor necrosis factor-alpha (TNF-α) were measured 120 min after reperfusion.
The metabolic rate of lactate in the group of asphyxial cardiac arrest rescued by ECLS was slow; therefore, the pH at 120 min after reperfusion was significantly lower in this group than that in the group of normal rats treated with ECLS. The hemodynamic data showed no between-group differences. The plasma levels of CRP, IL-6, IL-10, and TNF-α increased after ECLS treatment.
We successfully established a rodent ECLS model, which might be a useful approach for studying the pathophysiology induced by ECLS under clinical conditions.
Extracorporeal life support (ECLS) systems preserve adequate blood flow and oxygen supply in both adults [1, 2] and children [3, 4], and they have been described as life-saving devices. Because the components, such as a membrane oxygenator and catheters , and driving force of ECLS systems are nonbiological and nonphysiological, their effects on patients have not been completely elucidated. Studies are needed to elucidate the nonbiological and nonphysiological effects of ECLS. Although ECLS models in large animals, such as porcine models [6, 7], are well established because of the equipment, small animal models still have the potential for research in resuscitation studies. Rodent models are more economical when a high number of experiments must be performed. Furthermore, they provide various molecular tools such as genetic knockout or relevant diseases.
For the present study, we designed a rodent ECLS model for the following purposes: 1) to identify whether ECLS can be performed without a blood transfusion or the administration of drugs such as inotropes or vasopressors and by performing only crystalloid priming throughout the experiment; and 2) to measure the plasma levels of C-reactive protein (CRP), interleukin (IL)-6, IL-10, and tumor necrosis factor-α (TNF-α) 120 min after reperfusion.
Male Wistar–Kyoto rats (450–550 g) were randomly divided into a group of normal rats that did not receive ECLS (sham; n = 11), a group of normal rats treated with ECLS (NC + ECLS; n = 11) and a group of asphyxial cardiac arrest rats rescued by ECLS (CA + ECLS; n = 11). Two rats were housed per cage, and they had free access to Purina chow and water under a 12-h light–dark cycle. The experiments were conducted in accordance with the Guide for the Care and Use of Laboratory Animals, and the study protocol was approved by the Animal Care and Use Committee of National Taiwan University (IACUC number: 20140024).
Anesthesia and surgical preparation
Sodium pentobarbital (Sigma Chemical Co., St. Louis, MO, USA; 50 mg kg−1) was administered intraperitoneally to anesthetize the rats, and intravenous reinjections (35 mg kg−1) were performed every hour. The surgical sites (neck and right and left groin) were shaved, and the lungs were ventilated using a ventilator (Model 131, New England Medical Instruments, Medway, MA, USA). Ventilation was performed with room air through a 14G plastic catheter (B. Braun Medical, Bethlehem, PA, USA) at a tidal volume of 8 mL kg−1 and respiratory rate of 70 breaths per min. The rats were placed in the supine position and a rectal temperature probe (TP-K01 and TES-1300, TES Electrical Electronic Corp., Neihu Dist., Taipei, Taiwan) was inserted for the continuous monitoring of rectal temperature. Rectal temperature was maintained at 36 °C by using a circulating warm water blanket (B401H, Firstek Scientific Co. Ltd., Xinzhuang Dist, New Taipei City, Taiwan; TP22G, Gaymar Industries, Inc., Orchard Park, NY, 14,127 USA) and a heating lamp. The electrocardiogram (ECG) of lead II was recorded using a Gould ECG/Biotech amplifier (Gould Electronics, Cleveland, OH, USA).
The surgical sites were shaved and disinfected with betadine. The left femoral artery was cannulated using a Millar catheter (model SPC 320, size 2F; Millar Instrument, Houston, TX, USA) for the continuous monitoring of arterial pressure, and the left femoral vein was cannulated using PE-50 tubing for drug administration throughout the experiment. A 20G IV catheter (B. Braun Medical, Bethlehem, PA, USA) was inserted into the right femoral artery for ECLS inflow and arterial blood sampling. Cannulation of the right external jugular vein for ECLS venous outflow was then performed using a customized template-modified 5-hole 14G catheter, which was advanced to the junction of the right atrium and superior vena cava. This catheter was heparin-locked with 500 UI of heparin to prolong the activated clotting time to longer than 300 s. The arterial blood gas, arterial lactate, and hematocrit (i-STAT CG-4+ and 6+ cartridge, Abbott Point of Care, Princeton, NJ, USA) were determined at the baseline. The same procedure was used for all groups.
Cardiac arrest and ECLS
After instrumentation and the collection of the baseline parameters, a neuromuscular blockade was performed through the intravenous administration of pencuronium bromide (Sigma Chemical Co., St. Louis, MO, USA; 1 mg kg−1) for 5 min. Subsequently, the ventilator was switched off for 5 min to induce asphyxial cardiac arrest. Circulatory arrest was defined as a mean arterial blood pressure (MAP) of <20 mmHg because this pressure is commensurate with the cessation of all tissue blood flow.
The ECLS circuit (Fig. 1) designed for the rats consisted of an open venous reservoir (TERUMO®, Tokyo, Japan; 5-mL syringe), a membrane oxygenator (Micro-1 Rat Oxygenator, Dongguan Kewei Medical Instrument Co., Ltd., Guangdong, China), a heat exchanger (Radnoti Glass Technology Inc., Monrovia, CA, USA), silicone tubing (ID 1.6 mm), and a roller pump (Masterflex, Barrington, IL, USA) that was primed using 19–20 mL of Plasma-Lyte A (Baxter, Deerfield, IL, USA). After 5 min of asphyxia, reperfusion was initiated by starting the ECLS system, which was continued for 30 min.
Discontinuation of ECLS and intensive care unit phase
After 30 min of ECLS, the rats were weaned off the system by removing the right jugular catheter within 5 min, and the incision was closed in two layers by using an absorbable suture for the subcutaneous layer and skin. Arterial samples for blood gas analysis, hematocrit, and lactate were collected at 30, 60, 90, and 120 min after the commencement of reperfusion, defined as the start of ECLS. We did not perform a blood transfusion or administer drugs such as bicarbonate, inotropes, and vasopressors. The pump was primed only with crystalloid throughout the experiment. All rats were humanely euthanized at the end of the experiment.
Estimation of inflammatory response
To estimate the inflammatory response, plasma levels of CRP (Immunology Consultants Laboratory Inc., Portland, OR, USA), IL-6 (BioLegend Inc., San Diego, CA, USA), TNF-α (BioLegend Inc., San Diego, CA, USA), and IL-10 (Abcam, Cambridge, UK) were measured using enzyme-linked immunosorbent assay kits.
All data are reported as the mean ± standard error of the mean (SEM). The Mann–Whitney U test was used to compare differences between the groups at the same time points. Kaplan–Meier curves were plotted to depict the survival trend. The log-rank test was used to compare the risk between the two groups. In the mortality cases, the survival time was defined as the duration from the baseline to death; among the survivors, the duration was from the baseline to 120 min after the start of reperfusion. Statistical significance was defined as P < 0.05.
The Kaplan–Meier curves in Fig. 2 represent the cumulative survival of rats after reperfusion in the NC + ECLS and CA + ECLS groups. Figure 2 shows that not all rats were successfully resuscitated in the CA + ECLS group; therefore, the data for this group depend on the number of rats that survived until 30, 60, 90, or 120 min after reperfusion. Furthermore, the overall survival differed significantly between these two groups (P = 0.03).
Surgical preparation time and baseline parameters, namely animal body weight, arterial blood gas, hematocrit, and hemodynamic variables, did not differ between the NC + ECLS and CA + ECLS groups (Tables 1 and 2). The changes in arterial blood gas values after reperfusion during the experiments are presented in Table 2. The ion variable measurements before and after ECLS in the NC + ECLS and CA + ECLS groups are presented in the additional file (Additional file 1: Table S1). At 30 min after reperfusion, the hematocrit decreased to approximately half of the baseline value because the rats in both groups did not receive a blood transfusion during ECLS treatment. After the rats were weaned off the ECLS system, the retained solution in the circuit was reinfused into the rats in the intensive care unit phase; therefore, the hematocrit value gradually increased throughout the experiment. Simultaneously, the increase in MAP after reperfusion also depended on the reinfusion of the retained solution and not on the effects of inotrope or vasopressor administration. The lactate level, which reflects tissue perfusion, decreased in both groups but the decreasing rate was considerably more rapid in the NC + ECLS group (Fig. 3). All the basic parameters in the sham group (n = 2) are showed in the additional file (Additional file 2: Table S2).
Comparing the NC + ECLS and CA + ECLS groups at 120 min after reperfusion, the plasma levels of pro-inflammatory cytokines (i.e., IL-6, TNF-α, and CRP) and the anti-inflammatory cytokine (i.e., IL-10) were all significantly lower in the sham group than those in the NC + ECLS and CA + ECLS groups (Fig. 4). There showed no significant differences in IL-6, TNF-α, and CRP levels between NC + ECLS and CA + ECLS groups (Figs. 4a–c); however, the IL-10 levels differed significantly between these two groups (Fig. 4d).
In this study, we demonstrated that asphyxial cardiac arrest resuscitated using ECLS in rats was reproducible and enabled the survival of even the rats that were weaned off ECLS. However, the survival rate in the CA + ECLS group was only 63.6%, which was significantly lower than that in the NC + ECLS group. The mechanical-ventilation settings were similar to those in a previous study ; the only difference was the inspired gas. Because we used room air instead of higher inspired oxygen fraction as the inspired gas, the levels of blood gases (PaO2 and PaCO2) in our study were lower than those reported in previous studies [9,10,11,12]. However, the blood gas levels in the CA + ECLS and NC + ECLS groups did not exhibit any differences at the same time points in our study.
The rate of the decrease in lactate in the CA + ECLS group was lower than that in the NC + ECLS group. This might have caused the higher mortality rate and lower pH observed in the CA + ECLS group. The inability to metabolize lactate properly, which could cause acidosis in the body, might be a reason for the mortality rate in the CA + ECLS group . Therefore, the adjustment of the acid–base balance appears necessary.
The MAP levels in both groups exhibited their lowest value at 30 min after reperfusion and gradually increased during the remainder of the experiments. Ali et al. used phenylephrine (an α1 agonist) to augment MAP during the ECLS reperfusion  so did in others . By contrast, we used the remaining blood in the ECLS circuit rather than drugs to increase MAP in our study. This not only improves MAP but also could increase hematocrit levels.
In the CA + ECLS and NC + ECLS groups, hematocrit levels were significantly lower after reperfusion than at the baseline, because a blood transfusion was not performed during or after ECLS. Despite the decrease in hematocrit caused by hemodilution, sufficient oxygenation was maintained in both groups. Animal models have been reported to maintain adequate oxygen delivery to satisfy the whole body’s oxygen demands without blood product administration when the ventilator was supplied with higher inspired oxygen fraction [10, 12, 16,17,18]. However, we ventilated only with room air even in the absence of a blood transfusion to maintain acceptable oxygenation.
The results indicated that, compared with the sham group, the plasma levels of the inflammatory cytokines (i.e., IL-6, TNF-α, and CRP) and anti-inflammatory cytokine (i.e., IL-10) were significantly higher in the NC + ECLS and CA + ECLS groups after treatment with ECLS. In the previous study, Fujii et al. demonstrated that the inflammatory responses getting aggravated during ECLS perfusion . Herein, we suggested that a systemic inflammatory response continued to occur in our model even after the extracorporeal circulation was removed. Although the contact of blood with artificial surfaces , the nonpulsatile flow caused by the pump , and the nonlaminar flow during extracorporeal circulation  are possible factors responsible for the inflammatory response during ECLS, they cannot explain the inflammatory response sustained even after the rats were weaned off ECLS.
Extracorporeal circulation has been reported to disrupt the tight junctions of the small intestinal epithelial cells and cause gut barrier dysfunction. Then, because of this gut barrier dysfunction, bacteria in the intestinal lumen might translocate into the blood and increase the blood level of endotoxins such as lipopolysaccharide [22, 23]. This bacterial translocation might explain the inflammatory response even after the ECLS was disconnected in our study. In addition, the bacteria can not only induce an inflammatory response but also activate an anti-inflammatory response through stimulation of the vagus nerve . This might explaining the higher level of IL-10 in both ECLS treated groups compared with sham group.
The current model contains some still limitations. Because rodent blood volume is approximately 7% of body weight , it is difficult to measure the time dependent performances of the pro-inflammatory and anti-inflammatory cytokines after the rats are weaned off ECLS. The priming volume of our ECLS circuit was approximately 19–20 mL, which accounts for 61% of the blood volume of the rats. However, this was the minimum volume that we could attain in our model. Because we could not miniaturize the circuit, rats are the smallest model that we can operate with ECLS. Moreover, ECLS treatment time in clinical settings is considerably longer than that in our study, and the complex comorbidities seen in patients are difficult to mimic.
In this study, we successfully developed a novel and reproducible miniature rodent ECLS model without blood transfusion or drug administration. We also introduced asphyxial cardiac arrest to our ECLS model and resuscitated the rats successfully. However, the adjustment of the acid–base balance appears necessary, particularly in rats with induced asphyxial cardiac arrest. The proposed model might provide opportunities for studying the mechanism of the pathophysiology of cardiopulmonary bypass without drug effects, even in mutant rats.
Extracorporeal life support
Mean arterial blood pressure
Tumor necrosis factor-alpha
Chen YS, Lin JW, Yu HY, Ko WJ, Jerng JS, Chang WT, Chen WJ, Huang SC, Chi NH, Wang CH, et al. Cardiopulmonary resuscitation with assisted extracorporeal life-support versus conventional cardiopulmonary resuscitation in adults with in-hospital cardiac arrest: an observational study and propensity analysis. Lancet. 2008;372(9638):554–61.
Shin TG, Choi JH, Jo IJ, Sim MS, Song HG, Jeong YK, Song YB, Hahn JY, Choi SH, Gwon HC, et al. Extracorporeal cardiopulmonary resuscitation in patients with inhospital cardiac arrest: a comparison with conventional cardiopulmonary resuscitation. Crit Care Med. 2011;39(1):1–7.
Huang SC, Wu ET, Chen YS, Chang CI, Chiu IS, Wang SS, Lin FY, Ko WJ. Extracorporeal membrane oxygenation rescue for cardiopulmonary resuscitation in pediatric patients. Crit Care Med. 2008;36(5):1607–13.
Topjian AA, Nadkarni VM, Berg RA. Cardiopulmonary resuscitation in children. Curr Opin Crit Care. 2009;15(3):203–8.
Schaheen BW, Thiele RH, Isbell JM. Extracorporeal life support for adult cardiopulmonary failure. Best Pract Res Clin Anaesthesiol. 2015;29(2):229–39.
Salameh A, Dhein S. Strategies for pharmacological Organoprotection during extracorporeal circulation targeting ischemia-reperfusion injury. Front Pharmacol. 2015;6:296.
Keschenau PR, Ribbe S, Tamm M, Hanssen SJ, Tolba R, Jacobs MJ, Kalder J. Extracorporeal circulation increases proliferation in the intestinal mucosa in a large animal model. J Vasc Surg. 2016;64(4):1121–33.
Fujii Y, Shirai M, Inamori S, Takewa Y, Tatsumi E. Investigation of the biological effects of artificial perfusion using rat extracorporeal circulation model. Conf Proc IEEE Eng Med Biol Soc. 2014;2014:4483–6.
Boller M, Jung SK, Odegaard S, Muehlmatt A, Katz JM, Becker LB. A combination of metabolic strategies plus cardiopulmonary bypass improves short-term resuscitation from prolonged lethal cardiac arrest. Resuscitation. 2011;82(Suppl 2):S27–34.
Kim J, Yin T, Yin M, Zhang W, Shinozaki K, Selak MA, Pappan KL, Lampe JW, Becker LB. Examination of physiological function and biochemical disorders in a rat model of prolonged asphyxia-induced cardiac arrest followed by cardio pulmonary bypass resuscitation. PLoS One. 2014;9(11):e112012.
Dong GH, Xu B, Wang CT, Qian JJ, Liu H, Huang G, Jing H. A rat model of cardiopulmonary bypass with excellent survival. J Surg Res. 2005;123(2):171–5.
Funamoto M, Masumoto H, Takaori K, Taki T, Setozaki S, Yamazaki K, Minakata K, Ikeda T, Hyon SH, Sakata R. Green tea Polyphenol prevents diabetic rats from acute kidney injury after cardiopulmonary bypass. Ann Thorac Surg. 2016;101(4):1507–13.
Allen M. Lactate and acid base as a hemodynamic monitor and markers of cellular perfusion. Pediatr Crit Care Med. 2011;12(4 Suppl):S43–9.
Ali AA, Downey P, Singh G, Qi W, George I, Takayama H, Kirtane A, Krishnan P, Zalewski A, Freed D, et al. Rat model of veno-arterial extracorporeal membrane oxygenation. J Transl Med. 2014;12:37.
Engels M, Bilgic E, Pinto A, Vasquez E, Wollschlager L, Steinbrenner H, Kellermann K, Akhyari P, Lichtenberg A, Boeken U. A cardiopulmonary bypass with deep hypothermic circulatory arrest rat model for the investigation of the systemic inflammation response and induced organ damage. J Inflamm (Lond). 2014;11:26.
Zhu X, Ji B, Liu J, Sun Y, Wu S, Zheng Z, Long C, Tang Y. Establishment of a novel rat model without blood priming during normothermic cardiopulmonary bypass. Perfusion. 2014;29(1):63–9.
Liam BL, Plochl W, Cook DJ, Orszulak TA, Daly RC. Hemodilution and whole body oxygen balance during normothermic cardiopulmonary bypass in dogs. J Thorac Cardiovasc Surg. 1998;115(5):1203–8.
Cao HJ, Sun YJ, Zhang TZ, Zhou J, Diao YG. Penehyclidine hydrochloride attenuates the cerebral injury in a rat model of cardiopulmonary bypass. Can J Physiol Pharmacol. 2013;91(7):521–7.
Renne T, Schmaier AH, Nickel KF, Blomback M, Maas C. In vivo roles of factor XII. Blood. 2012;120(22):4296–303.
O'Neil MP, Fleming JC, Badhwar A, Guo LR. Pulsatile versus nonpulsatile flow during cardiopulmonary bypass: microcirculatory and systemic effects. Ann Thorac Surg. 2012;94(6):2046–53.
Cunningham KS, Gotlieb AI. The role of shear stress in the pathogenesis of atherosclerosis. Lab Investig. 2005;85(1):9–23.
Kurundkar AR, Killingsworth CR, McIlwain RB, Timpa JG, Hartman YE, He D, Karnatak RK, Neel ML, Clancy JP, Anantharamaiah GM, et al. Extracorporeal membrane oxygenation causes loss of intestinal epithelial barrier in the newborn piglet. Pediatr Res. 2010;68(2):128–33.
McILwain RB, Timpa JG, Kurundkar AR, Holt DW, Kelly DR, Hartman YE, Neel ML, Karnatak RK, Schelonka RL, Anantharamaiah GM, et al. Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related SIRS due to the release of preformed stores in the intestine. Lab Investig. 2010;90(1):128–39.
Sundman E, Olofsson PS. Neural control of the immune system. Adv Physiol Educ. 2014;38(2):135–9.
Lee HB, Blaufox MD. Blood volume in the rat. J Nucl Med. 1985;26(1):72–6.
R.W.C. and C.H.W. developed the concept, designed the study, and wrote the manuscript. R.W.C. performed the animal experiments, collected the data, and performed the statistical analysis. C.M.L., H.Y.Y., and Y.S.C. provided funding and advice on the surgical procedure. C.M.L. and H.Y.Y. participated in the data interpretation. C.H.W. supervised this work and critically revised the manuscript. All authors read and approved the final manuscript.
Consent for publication
This study was supported by grants from the National Science Council of Taiwan (MOST 102–2314-B-002-169-MY2), National Taiwan University Hospital, Hsin-Chu Branch (105-HCH018; 105-HCH060), National Taiwan University Hospital (106–16).
Availability of data and materials
The datasets used and analyzed in the current study are available from the corresponding author upon reasonable request.
The authors have no conflicts of interest relevant to this article.
The experiments were conducted in accordance with the Guide for the Care and Use of Laboratory Animals, and the study protocol was approved by the Animal Care and Use Committee of National Taiwan University (IACUC number: 20140024).
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Cite this article
Chang, RW., Luo, CM., Yu, HY. et al. Investigation of the pathophysiology of cardiopulmonary bypass using rodent extracorporeal life support model. BMC Cardiovasc Disord 17, 123 (2017). https://doi.org/10.1186/s12872-017-0558-6
- Asphyxial cardiac arrest
- Extracorporeal life support
- Inflammatory response
- Rat model