Effects of non-supervised low intensity aerobic excise training on the microvascular endothelial function of patients with type 1 diabetes: a non-pharmacological interventional study
BMC Cardiovascular Disorders volume 16, Article number: 23 (2016)
The aim of the present study was to evaluate changes in microvascular density and reactivity in patients with type 1 diabetes (T1D) resulting from low intensity chronic exercise training.
This study included 22 (34 ± 7 years) consecutive outpatients with T1D and disease duration > 6 years. We used intravital video-microscopy to measure basal skin capillary density and capillary recruitment using post-occlusive reactive hyperemia (PORH) in the dorsum of the fingers. Endothelium-dependent and -independent vasodilation of the skin microcirculation was evaluated in the forearm with a laser Doppler flow monitoring (LDF) system in combination with acetylcholine and sodium nitroprusside iontophoresis, PORH and local thermal hyperemia.
The basal mean capillary density (MCD) after exercise training was significantly higher than before exercise (134 ± 25 vs. 119 ± 19 capillaries/mm2, respectively; P = 0.0013). MCD during PORH was also higher after exercise (140 ± 26 vs. 121 ± 24 capillaries/mm2, respectively; P < 0.0001). Endothelium-dependent capillary recruitment during PORH was also significantly higher after exercise (140 ± 26 vs. 134 ± 25 capillaries/mm2, respectively; P < 0.0012). There were no significant changes in skin microvascular reactivity after exercise as investigated using LDF.
Our results showed that low intensity aerobic exercise, performed four times per week for 12 weeks by patients with T1D, induces significant increases in microvascular density and endothelial-dependent capillary reactivity.
ClinicalTrials.gov NCT02441504. Registered 7 May 2015.
Type 1 diabetes mellitus (T1D) is an autoimmune disease whose clinical manifestations are characterized by extensive damage to the macro and microcirculation. It is believed that the loss of glycemic control that occurs during the disease is responsible for functional and structural vascular alterations, contributing to significant increases in the risk of morbidity and mortality in this group of individuals [1–4].
Through the induction of oxidative stress, inflammation and the formation of nonenzymatic advanced glycation end products (AGEs), hyperglycemia can cause microvascular abnormalities that impair tissue nutrition [5, 6]. In this context, from the beginning of the disease, patients with T1D present with widespread precapillary vasodilation and tissue hypoxia, which promotes compensatory increases in the level of vasoactive substances and chronic elevation of the microvascular blood flow, resulting in morphological and functional changes associated with capillary rarefaction [7–12].
In spite of the positive effects of anti-hyperglycemic therapy  and drugs that attenuate inflammation and oxidative stress , chronic aerobic exercise has been shown to exert anti-inflammatory, anti-hyperglycemic and anti-oxidant effects . Moreover, exercise training contributes to an increase in capillary density in patients with T1D . In fact, aerobic training is often incorporated into T1D treatment therapies [17–20] because it can prevent undesirable morphological changes and, through the processes of arteriogenesis and angiogenesis, restore and expand the vascular reactivity and tissue perfusion that are otherwise impaired by the disease [21–24].
Several studies have demonstrated that aerobic training with intensity exceeding 50 % of VO2max can improve macro- and microvascular endothelial function in young people with T1D [25–27], suggesting that the microvascular function in patients with T1D is directly proportional to the individual level of aerobic fitness . However, it is still not clear whether the effects of low intensity exercise intervention, which is considered to be safer than higher intensity intervention, are more suited to the average functional capacity of this population. Considering the therapeutic potential of aerobic training to promote metabolic and vascular adaptations that prevent the development of clinical complications in T1D patients, this study aimed to investigate the effects of 12 weeks of low intensity aerobic exercise on microvascular reactivity in this group of individuals.
The present study was performed in accordance with the Helsinki Declaration of 1975, as revised in 2000, and the Institutional Review Board (IRB) of the University Hospital of the State University of Rio de Janeiro, Brazil approved of this study. Once considered eligible, all subjects read and signed an informed consent document that was approved by the IRB.
Study subjects presenting with type 1 diabetes (diagnosis based on a typical clinical presentation as well as the need to use insulin continuously since the diagnosis) of both sexes were recruited among patients who were followed up at a public hospital. Twenty-eight individuals with T1D diagnosed for more than 6 years, between 25 and 50 years of age, were recruited for the study. Twenty-two individuals (mean age 34 ± 7 years) completed the exercise protocol. There were 13 males (60 %) and 9 females (40 %) in the study group. The other six subjects (21 % of the subjects initially recruited for the study) left the study due to personal reasons.
The initial clinical evaluation included the patient’s history and physical examination as well as recording of the following anthropometric data: weight, height, waist circumference and body mass index (BMI). Blood pressure measurements were collected with patients in the supine position after 5 min in quiet surroundings; they were repeated twice with a two-minute interval between measurements. All measurements were performed before and after 12 weeks of physical training.
All patients included in the study were advised to maintain the same level of usual daily physical activity during the experimental protocol. Moreover, a regular diet for patients with type 1 diabetes was prescribed and supervised by the clinical staff of the tertiary care setting where they were recruited to certify that no significant changes occurred in the composition or total calories of the diet.
The diabetic patients included in the study were relatively young and did not present any co-morbidity. They were treated exclusively with insulin for diabetes control by an endocrinologist in the tertiary care setting, and none of them was taking other medications.
Blood sampling and laboratory tests
On the morning scheduled for the evaluation of cutaneous microcirculation, the patients presented in 12-h fasted condition for blood collection. Smokers should not have smoked or ingested caffeine from the night before until the completion of the tests. The following variables were measured: fasting and postprandial plasma glucose, total cholesterol, LDL and HDL cholesterol, triglycerides, transaminases, high-sensitivity C-reactive protein, gamma-glutamyl transferase, creatine kinase, urea, creatinine, albumin and uric acid by colorimetric reactions by using a Cobas Mira-machine (Roche). Blood samples were collected before and after the physical training period. LDL cholesterol was calculated using Friedewald’s formula. Serum samples were kept frozen at –80 °C until measurement of interleukin-6 (IL-6) levels with a commercial ELISA kit (Cayman Chemical Company, Ann Arbor, MI, USA) according to the manufacturer’s instructions.
The study participants followed a non-supervised aerobic training protocol targeted at low intensity and corresponding to 40 % of their heart rate (HR) reserve (HRR). Resting HR was evaluated at the time of assessment of microvascular reactivity, and maximum HR was calculated using the formula [208 – (0.7 × age)] as described by Tanaka and colleagues . HRR was calculated as proposed by Karvonen  [(HRmax – HRrest) x 40 % + HRrest] because it is highly correlated with VO2 maximum . The patients were advised to perform exercise sessions four times per week for 12 weeks that included alternating walking and running, in accordance with the patient’s fitness level, corresponding to 40 % of the HRR. Follow-up of the training period was conducted through weekly telephone contact. Heart rate was monitored using heart rate monitors (Polar Electro Oy, Kempele, Finland). During the first 4 weeks of training, 10 min were added per session every week to promote gradual progression in the range from 30 to 60 min during the remaining 8 weeks.
Capillaroscopy by intra-vital videomicroscopy
Microcirculatory tests were always performed after a 20-min period of rest in the supine position in a temperature-controlled room (23 ± 1 °C), thus excluding the influence of climate conditions from the results of the tests.
The capillary density, defined as the number of perfused capillaries per mm2 of skin area, was assessed by high-resolution intra-vital color microscopy (Moritex, Cambridge, UK) using a video microscopy system with an epi-illuminated fiber optic microscope containing a 100-W mercury vapor lamp light source and an M200 objective with a final magnification of 200×. The dorsum of the non-dominant middle phalanx was used for image acquisition, which occurred while the patient sat comfortably in a constant temperature environment (23 ± 1 °C), as described in detail by Antonios et al. [32–34]. Images were acquired and saved for posterior off-line analysis using a semi-automatic integrated system (Microvision Instruments, Evry, France). The mean capillary density was calculated as the arithmetic mean of the number of visible (i.e., spontaneously perfused) capillaries in three contiguous microscopic fields of 1 mm2 each. A blood pressure cuff was then applied to the patient’s arm and inflated to suprasystolic pressure (50 mmHg above systolic arterial pressure) to completely interrupt blood flow for three minutes (testing post-occlusive reactive hyperemia, PORH). After cuff release, images were again acquired and recorded during the following 60–90 s, during which a maximal hyperemic response was expected to occur.
We assessed the variability of skin video capillaroscopy in a previous study . The reproducibility of this methodology was assessed by examining an identical area of the finger skin; the intra-observer repeatability of data analysis was assessed by reading the same images in a blinded manner on two separate occasions (n = 20; coefficient of variability, 4.3 %). To assess inter-observer repeatability, a second observer independently assessed the capillary density in the same images (n = 15; coefficient of variability, 3.3 %).
Microvascular reactivity to pharmacological stimulation
Microvascular cutaneous reactivity was studied by laser Doppler flow monitoring (LDF), a method that has previously been standardized and validated [10, 35, 36]. Real-time variations in cutaneous microcirculatory flow were assessed through an LDF system (wavelength 780 nm; Periflux 5001, Perimed AB, Järfälla, Sweden) attached to a pharmacological micro-iontophoresis system (PeriIont, Perimed AB). The iontophoresis microelectrodes (PF 383, Perimed) were incorporated into the head of a laser probe (PF 481–1, Perimed), and the probe temperature was standardized to 32°C to avoid variations in skin temperature and, consequently, in the measurements of microvascular flow. The drug-delivery electrodes were filled with 200 μl of 1 % ACh solution (Sigma Chemical Co., USA) and placed on the ventral surface of the forearm, away from visible subcutaneous veins and areas of skin pigmentation. Neutral electrodes for current dispersion were placed 15 cm above the infusion electrodes, and reference points were marked and annotated to ensure reproducibility during the second examination, which took place at the end of the intervention period. After measuring the baseline flow for 5 min, four equal cumulative doses of ACh (anodal current) were applied at a constant intensity of 0.1 mA for 10, 20, 40 and 80 s, with total charges of 1, 2, 4 and 8 millicoulombs, respectively, allowing for a 120-s interval between doses. Recording of the microvascular flow induced by ACh was conducted for 10 min as follows: 2 min for each dose and 2 min to allow the flow to reach a plateau after the last dose. Using a new delivery electrode applied to a different location on the same forearm, four doses of a solution of 1 % sodium nitroprusside (SNP; Sigma Chemical CO, USA) dissolved in distilled water were delivered using a cathodal current (same charges and intervals as for ACh).
The laser Doppler output, which is semiquantitative, is expressed in arbitrary perfusion units (PUs) of output voltage (1 PU = 10 mV) in accordance with general consensus (European Laser Doppler Users Groups, London, 1992). An area under the flow response to ACh curve could be defined using the PeriSoft for Windows 2.5 software (Perimed, Järfälla, Sweden); this area was quantitatively measured and expressed in PU/s.
Microvascular reactivity to physiologic stimulation
After measuring the resting capillary flow for 5 min using another laser probe (PF 457, Perimed) that had been positioned at the start of the recording session, the PORH test was performed on the same forearm of pharmacological stimulation. Following release of the pressure, the maximum flow and area under the PORH curve were measured. The mean value of the resting flow was considered the basal flow value. When the microvascular flow returned to its basal value after the PORH test (typically 5–10 min), the maximal skin microvascular vasodilatation was investigated with prolonged (20 min) local heating of the laser probe to 42°C. The baseline microvascular flux value was calculated as described above.
The values were expressed as the means ± standard error of the means for variables with a normal distribution and as median (percentiles 25th–75th) for variables with a non-parametric distribution according to results of the Shapiro-Wilk normality test. The data were analyzed by two-tailed paired t tests or the two-tailed Wilcoxon signed-rank test as appropriate. The null hypothesis was rejected at P < 0.05.
The clinical and laboratory variables of the patients at baseline and post-exercise training are presented in Table 1. We observed significant reductions in body weight and body mass index, as well as in uric acid and interleukin-6 serum levels, after 12 weeks of exercise training.
The mean capillary density after exercise training was significantly higher than before training (Fig. 1). A similar pattern was found during the PORH test; the capillary density was significantly higher after the training period compared with before the training period (Fig. 1). The increase in the capillary density measured in the basal state was from 119 ± 19 to 134 ± 25 capillaries/mm2 (P = 0.0013) and after PORH from 121 ± 24 to 140 ± 26 capillaries/mm2 (P < 0.0001). Moreover, there was improvement in the capillary recruitment induced by the PORH test after training; the capillary density after the PORH test increased from 134 ± 25 to 140 ± 26 capillaries/mm2 (P = 0.0012).
Microvascular reactivity to pharmacological and physiological stimuli
The skin microvascular vasodilation responses induced by either endothelial-dependent (ACh) or endothelial-independent (SNP) vasoactive drugs were not different before and after exercise training (Fig. 2). The same pattern of responses was observed after physiological endothelial-dependent stimulation with the PORH test and local heating (Fig. 2). The individual values of microvascular parameters obtained with LDF are presented in Additional file 1.
The results of the present study indicate that 3 months of non-supervised aerobic exercise training performed at 40 % of HRR significantly increased the microvascular density of T1D patients and reduced their BMI and uric acid serum levels. The low intensity aerobic training program also improved basal capillary density and capillary recruitment during post-occlusive reactive hyperemia, suggesting that improvements occurred in systemic capillary number and perfusion.
It is well demonstrated that elevated uric acid serum levels are associated with endothelial dysfunction and renal microvascular complications in patients with T1D [36–39]. Although the patients’ serum uric acid levels were within the normal range, low-intensity aerobic training was able to promote a significant reduction that could be associated with increased capillary density. Such changes may have contributed to expanding the supply of tissue nutrients and reducing the catabolism of adenine as well as the rate of uric acid formation [40, 41].
Despite previous contradictory reports [42, 43], our results showed that aerobic activity of low difficulty level, performed within the functional capacity of sedentary individuals, can promote significant increases in the microvascular density of T1D patients, who appear to have a severely compromised angiogenic process in the absence of exercise [44, 45]. In fact, as shown by previous studies [12, 46], and considering that sedentary T1D patients do not present with a capillary reserve in either the hands or feet , the changes in capillary density observed in the present study after exercise training suggest that low intensity aerobic exercise not only represents an important stimulus for increased capillary perfusion, it also contributes to increasing the number of capillaries.
In our study, the improvement in capillary perfusion and function was not accompanied by significant changes in microvascular reactivity after exercise training. It is conceivable that the low intensity exercise training did not induce sufficient increases in blood flow velocity and vascular shear stress required to significantly increase the release and bioavailability of vasoactive molecules, such as nitric oxide. In fact, higher intensity physical exercise has already been shown to induce significant increases in cutaneous microvascular reactivity [47, 48]. On the other hand, vascular growth and remodeling and enhanced angiogenesis, i.e., capillary growth, are well-known features of physiological adaptations to chronic exercise and have been extensively reviewed [24, 49].
Low-to-moderate intensity aerobic exercise has been associated with lower oxidative damage to the vascular endothelium [50–52] as well as with selective increases in angiogenic factors such as vascular endothelial growth factor (VEGF) and hypoxia-inducible factor (HIF-1α) [42, 53]. On the other hand, it is possible that the average intensity of 40 % of the heart rate reserve used in our study corresponds to an increase in the ventricular ejection fraction that is not associated with high sympathetic activation, which could have beneficial effects on capillary perfusion without imposing harmful interference associated with increases in arteriolar tone. Therefore, it is reasonable to speculate that during each workout, there was an increase in the supply of humoral factors, such as atrial natriuretic peptide, that are capable of stimulating perfusion and/or increasing capillary density . We speculate this in spite of the absence of a training effect on endothelium-dependent or -independent microvascular reactivity, as measured by laser Doppler flowmetry, which is most likely caused by insufficient increases in the flow velocity and vascular shear stress needed to increase the bioavailability of vasoactive molecules such as nitric oxide . The absence of significant differences in microvascular reactivity, as measured by single point laser Doppler methodology, could be related to the high variability of the vasodilator effects obtained upon cutaneous iontophoresis with acetylcholine and sodium nitroprusside.
In this context, there is evidence that an increase in plasma free fatty acid (FFA) levels, which predominantly occurs with exercise intensity below 50 % of VO2max [56–58], can activate peroxisome proliferator-activated receptor beta or delta (PPAR-β or PPAR-δ), as well as PPAR-δ coactivator-alpha (PGC-1α), contributing to the expression of VEGF and consequent development of the angiogenic process [59–61]. Nevertheless, we do not have data about plasma levels of VEGF or of its soluble receptor FLT-1 in the patients with T1D, and thus we cannot conclude that this mechanism is involved in the increases in systemic capillary observed after exercise training.
On the other hand, it has been suggested in animal studies that low intensity exercise can activate PGC-1α [62, 63] independently of 5′ adenosine monophosphate-activated protein kinase (AMPK) activation, which might only occur with exercise of higher intensity .
Increases in body temperature have been associated with improvements in tissue perfusion and capillary angiogenesis in animals and humans [65, 66]. In our study, the average temperature of the T1D patients during exercise was 37.8 ± 0.8°C, which may have been an important stimulus for the changes in capillary perfusion. In this sense, because it has been shown that physiological levels of urate are needed to promote increases in capillary perfusion in thermal stress situations , it is reasonable to speculate that the decrease in uric acid levels represents improvements in perfusion associated with the adaptive effects of low intensity training and resulting in capillary angiogenic processes.
Our results also indicated that low intensity aerobic training was able to restore the ability of capillary self-regulation at rest. In fact, studies with diabetic animals had already suggested that, through stimulating angiogenesis, aerobic training was able to partially restore the capillary diffusion capacity and regional distribution reduced by hyperglycemia  and losses in capillary perfusion [44, 68, 69].
Training methodologies that combine low intensity aerobic exercise in situations that mimic the reduction of tissue nutrients present in patients with T1D, such as venous occlusion training techniques, have been shown to result in significant metabolic adaptations associated with improvements in insulin sensitivity and muscle strength . Moreover, despite the proposition that high intensity interval training can promote increases in the capillary density of skeletal muscle, this exercise intensity has recently been suggested to be small compared to lower exercise intensities [71, 72].
It is well established that interplay between inflammatory and metabolic alterations leads to vascular injury in diabetes, which is mainly represented by microvascular endothelial dysfunction [73–75]. Therefore, an array of circulating biomarkers of systemic subclinical chronic inflammation has been investigated to target therapy in diabetes. Interleukin 6 (IL-6) appears to be a systemic inflammatory marker that is correlated with the degree of inflammatory activity in both T1D and T2D [74, 76]. In our study, plasma IL-6 levels were markedly and significantly reduced after exercise, indicating that low intensity aerobic exercise is an efficient non-pharmacologic intervention affecting the vascular inflammatory profile of T1D patients. This effect could also explain the improvement in endothelium-dependent capillary recruitment observed after exercise training.
It is important to note that in addition to its well-known pro-inflammatory actions, IL-6 can also have an anti-inflammatory role. Multiple cytokines, including IL-6, IL-1, and TNF-α, are consistently elevated in inflammatory states and have been recognized as targets of therapeutic intervention. Nevertheless, IL-6 can also play an anti-inflammatory role in both local and systemic inflammatory responses by controlling levels of the pro-inflammatory cytokines TNF-α and IL-1 . In our study, the reductions in IL-6 plasma levels observed after exercise training suggests that this non-pharmacologic intervention is able to reduce the low-grade systemic inflammation that is typical of T1D patients. In future studies, comprehensive evaluations of plasma levels of different pro- and anti-inflammatory cytokines are warranted to investigate the putative interactions between these different cytokines.
Limitations and strengths of the study
The present study has limitations that may have influenced the findings. Such limitations include the fact that there was no control (sedentary) group of patients. Considering that physical exercise is a classical non-pharmacologic intervention in cardiovascular and metabolic diseases, we consider that it could be unethical to have a group of patients not participating in the exercise training program. On the other hand, we could have used a control (run in) period when the patients could have been evaluated after a period of inactivity. However, the main drawback of this type of experimental design is the resulting decrease of adherence to the study protocol, which is typical of patients with chronic diseases, and could have resulted in a greater dropout rate from the study.
Finally, it is also worth mentioning that the increases in systemic capillarity and improvement of capillary recruitment in patients with type 1 diabetes were obtained with an exercise training protocol that can be easily performed in the everyday life of patients with chronic diseases.
Our results showed that non-supervised low intensity aerobic exercise, performed four times per week for 12 weeks by patients with type 1 diabetes, induces significant increases in microvascular density and endothelial-dependent capillary reactivity. Microvascular improvement in patients with diabetes is essential for preventing complications and targeting end-organ damage.
Advanced glycation end products
Body mass index
High-density lipoprotein cholesterol
High-sensitivity C-reactive protein
Low-density lipoprotein cholesterol
Laser Doppler flow monitoring
Mean capillary density, PORH, Post-occlusive reactive hyperemia
Type 1 diabetes
Delbin MA, Trask AJ. The diabetic vasculature: physiological mechanisms of dysfunction and influence of aerobic exercise training in animal models. Life Sci. 2014;102:1–9.
Chao CY, Cheing GL. Microvascular dysfunction in diabetic foot disease and ulceration. Diabetes Metab Res Rev. 2009;25:604–14.
Curtis TM, Gardiner TA, Stitt AW. Microvascular lesions of diabetic retinopathy: clues towards understanding pathogenesis? Eye. 2009;23:1496–508.
Khan F, Elhadd TA, Greene SA, Belch JJ. Impaired skin microvascular function in children, adolescents, and young adults with type 1 diabetes. Diabetes Care. 2000;23:215–20.
Levy BI, Schiffrin EL, Mourad JJ, Agostini D, Vicaut E, Safar ME, et al. Impaired tissue perfusion: a pathology common to hypertension, obesity, and diabetes mellitus. Circulation. 2008;118:968–76.
Das Evcimen N, King GL. The role of protein kinase C activation and the vascular complications of diabetes. Pharmacol Res. 2007;55:498–510.
Tooke JE. Microvascular function in human diabetes. A physiological perspective. Diabetes. 1995;44:721–6.
Candido R, Allen TJ. Haemodynamics in microvascular complications in type 1 diabetes. Diabetes Metab Res Rev. 2002;18:286–304.
Hwu CM, Lin KH. Uric acid and the development of hypertension. Med Sci Monit. 2010;16:RA224–30.
Gomes MB, Matheus AS, Tibirica E. Evaluation of microvascular endothelial function in patients with type 1 diabetes using laser-Doppler perfusion monitoring: which method to choose? Microvasc Res. 2008;76:132–3.
Tibirica E, Rodrigues E, Cobas R, Gomes MB. Increased functional and structural skin capillary density in type 1 diabetes patients with vascular complications. Diabetol Metab Syndr. 2009;1:24.
Tibirica E, Rodrigues E, Cobas RA, Gomes MB. Endothelial function in patients with type 1 diabetes evaluated by skin capillary recruitment. Microvasc Res. 2007;73:107–12.
Miyazaki C, Takeuchi M, Yoshitani H, Otani S, Sakamoto K, Yoshikawa J. Optimum hypoglycemic therapy can improve coronary flow velocity reserve in diabetic patients: demonstration by transthoracic doppler echocardiography. Circ J. 2003;67:945–50.
Marketou ME, Zacharis EA, Koukouraki S, Stathaki MI, Arfanakis DA, Kochiadakis GE, et al. Effect of angiotensin-converting enzyme inhibitors on systemic inflammation and myocardial sympathetic innervation in normotensive patients with type 2 diabetes mellitus. J Hum Hypertens. 2008;22:191–6.
Petersen AM, Pedersen BK. The anti-inflammatory effect of exercise. J Appl Physiol (1985). 2005;98:1154–62.
Shono N, Urata H, Saltin B, Mizuno M, Harada T, Shindo M, et al. Effects of low intensity aerobic training on skeletal muscle capillary and blood lipoprotein profiles. J Atheroscler Thromb. 2002;9:78–85.
Galassetti P, Riddell MC. Exercise and type 1 diabetes (T1DM). Compr Physiol. 2013;3:1309–36.
MacMillan F, Kirk A, Mutrie N, Matthews L, Robertson K, Saunders DH. A systematic review of physical activity and sedentary behavior intervention studies in youth with type 1 diabetes: study characteristics, intervention design, and efficacy. Pediatr Diabetes. 2014;15:175–89.
Froisland DH, Graue M, Markestad T, Skrivarhaug T, Wentzel-Larsen T, Dahl-Jorgensen K. Health-related quality of life among Norwegian children and adolescents with type 1 diabetes on intensive insulin treatment: a population-based study. Acta Paediatr. 2013;102:889–95.
Kennedy A, Nirantharakumar K, Chimen M, Pang TT, Hemming K, Andrews RC, et al. Does exercise improve glycaemic control in type 1 diabetes? A systematic review and meta-analysis. PLoS One. 2013;8:e58861.
Garber CE, Blissmer B, Deschenes MR, Franklin BA, Lamonte MJ, Lee IM, et al. American College of Sports Medicine position stand. Quantity and quality of exercise for developing and maintaining cardiorespiratory, musculoskeletal, and neuromotor fitness in apparently healthy adults: guidance for prescribing exercise. Med Sci Sports Exerc. 2011;43:1334–59.
Herbst A, Kordonouri O, Schwab KO, Schmidt F, Holl RW. Impact of physical activity on cardiovascular risk factors in children with type 1 diabetes: a multicenter study of 23,251 patients. Diabetes Care. 2007;30:2098–100.
Manders RJ, Van Dijk JW, van Loon LJ. Low-intensity exercise reduces the prevalence of hyperglycemia in type 2 diabetes. Med Sci Sports Exerc. 2010;42:219–25.
Prior BM, Yang HT, Terjung RL. What makes vessels grow with exercise training? J Appl Physiol (1985). 2004;97:1119–28.
Roche DM, Edmunds S, Cable T, Didi M, Stratton G. Skin microvascular reactivity in children and adolescents with type 1 diabetes in relation to levels of physical activity and aerobic fitness. Pediatr Exerc Sci. 2008;20:426–38.
Seeger JP, Thijssen DH, Noordam K, Cranen ME, Hopman MT, Nijhuis-van der Sanden MW. Exercise training improves physical fitness and vascular function in children with type 1 diabetes. Diabetes Obes Metab. 2011;13(4):382–4.
Fuchsjager-Mayrl G, Pleiner J, Wiesinger GF, Sieder AE, Quittan M, Nuhr MJ, et al. Exercise training improves vascular endothelial function in patients with type 1 diabetes. Diabetes Care. 2002;25:1795–801.
Roche DM, Edmunds S, Cable T, Didi M, Stratton G. Skin microvascular reactivity in children and adolescents with type 1 diabetes in relation to levels of physical activity and aerobic fitness. Pediatr Exerc Sci. 2008;20:426–38.
Tanaka H, Monahan KD, Seals DR. Age-predicted maximal heart rate revisited. J Am Coll Cardiol. 2001;37:153–6.
Karvonen MJ, Kentala E, Mustala O. The effects of training on heart rate; a longitudinal study. Ann Med Exp Biol Fenn. 1957;35:307–15.
Miller WC, Wallace JP, Eggert KE. Predicting max HR and the HR-VO2 relationship for exercise prescription in obesity. Med Sci Sports Exerc. 1993;25:1077–81.
Antonios TF, Kaski JC, Hasan KM, Brown SJ, Singer DR. Rarefaction of skin capillaries in patients with anginal chest pain and normal coronary arteriograms. Eur Heart J. 2001;22:1144–8.
Nama V, Manyonda IT, Onwude J, Antonios TF. Structural capillary rarefaction and the onset of preeclampsia. Obstet Gynecol. 2012;119:967–74.
Antonios TF, Nama V, Wang D, Manyonda IT. Microvascular remodelling in preeclampsia: quantifying capillary rarefaction accurately and independently predicts preeclampsia. Am J Hypertens. 2013;26:1162–9.
Kaiser SE, Sanjuliani AF, Estato V, Gomes MB, Tibirica E. Antihypertensive treatment improves microvascular rarefaction and reactivity in low-risk hypertensive individuals. Microcirculation. 2013;20:703–16.
Matheus AS, Tibirica E, da Silva PB, de Fatima Bevilacqua da Matta M, Gomes MB. Uric acid levels are associated with microvascular endothelial dysfunction in patients with Type 1 diabetes. Diabet Med. 2011;28:1188–93.
Hovind P, Rossing P, Johnson RJ, Parving HH. Serum uric acid as a new player in the development of diabetic nephropathy. J Ren Nutr. 2011;21:124–7.
Edwards NL. The role of hyperuricemia in vascular disorders. Curr Opin Rheumatol. 2009;21:132–7.
Gersch C, Palii SP, Kim KM, Angerhofer A, Johnson RJ, Henderson GN. Inactivation of nitric oxide by uric acid. Nucleosides Nucleotides Nucleic Acids. 2008;27:967–78.
Hayden MR, Tyagi SC. Uric acid: A new look at an old risk marker for cardiovascular disease, metabolic syndrome, and type 2 diabetes mellitus: The urate redox shuttle. Nutr Metab (Lond). 2004;1:10.
Gagliardi AC, Miname MH, Santos RD. Uric acid: A marker of increased cardiovascular risk. Atherosclerosis. 2009;202:11–7.
Tang XY, Hong HS, Chen LL, Lin XH, Lin JH, Lin Z. Effects of exercise of different intensities on the angiogenesis, infarct healing, and function of the left ventricle in postmyocardial infarction rats. Coron Artery Dis. 2011;22:497–506.
Schantz P, Henriksson J, Jansson E. Adaptation of human skeletal muscle to endurance training of long duration. Clin Physiol. 1983;3:141–51.
Kivela R, Silvennoinen M, Touvra AM, Lehti TM, Kainulainen H, Vihko V. Effects of experimental type 1 diabetes and exercise training on angiogenic gene expression and capillarization in skeletal muscle. FASEB J. 2006;20:1570–2.
Katz MA, McNeill G. Defective vasodilation response to exercise in cutaneous precapillary vessels in diabetic humans. Diabetes. 1987;36:1386–96.
Tibirica E, Rodrigues E, Cobas R, Gomes MB. Impairment of skin capillary recruitment precedes chronic complications in patients with type 1 diabetes. Rev Diabet Stud. 2007;4:85–8.
Lenasi H, Strucl M. Effect of regular physical training on cutaneous microvascular reactivity. Med Sci Sports Exerc. 2004;36:606–12.
Mitranun W, Deerochanawong C, Tanaka H, Suksom D. Continuous vs interval training on glycemic control and macro- and microvascular reactivity in type 2 diabetic patients. Scand J Med Sci Sports. 2014;24:e69–76.
Duncker DJ, Bache RJ. Regulation of coronary blood flow during exercise. Physiol Rev. 2008;88:1009–86.
Di Francescomarino S, Sciartilli A, Di Valerio V, Di Baldassarre A, Gallina S. The effect of physical exercise on endothelial function. Sports Med. 2009;39:797–812.
Gustafsson T. Vascular remodelling in human skeletal muscle. Biochem Soc Trans. 2011;39:1628–32.
Thorin E, Thorin-Trescases N. Vascular endothelial ageing, heartbeat after heartbeat. Cardiovasc Res. 2009;84:24–32.
Kondo H, Fujino H, Murakami S, Tanaka M, Kanazashi M, Nagatomo F, et al. Low-intensity running exercise enhances the capillary volume and pro-angiogenic factors in the soleus muscle of type 2 diabetic rats. Muscle Nerve. 2015;51:391–9.
Morvan E, Lima NE, Machi JF, Mostarda C, De Angelis K, Irigoyen MC, et al. Metabolic, hemodynamic and structural adjustments to low intensity exercise training in a metabolic syndrome model. Cardiovasc Diabetol. 2013;12:89.
Tjonna AE, Lee SJ, Rognmo O, Stolen TO, Bye A, Haram PM, et al. Aerobic interval training versus continuous moderate exercise as a treatment for the metabolic syndrome: a pilot study. Circulation. 2008;118:346–54.
Achten J, Jeukendrup AE. Optimizing fat oxidation through exercise and diet. Nutrition. 2004;20:716–27.
De Feo P, Di Loreto C, Lucidi P, Murdolo G, Parlanti N, De Cicco A, et al. Metabolic response to exercise. J Endocrinol Invest. 2003;26:851–4.
Wolfe RR. Fat metabolism in exercise. Adv Exp Med Biol. 1998;441:147–56.
Silvennoinen M, Rinnankoski-Tuikka R, Vuento M, Hulmi JJ, Torvinen S, Lehti M, et al. High-fat feeding induces angiogenesis in skeletal muscle and activates angiogenic pathways in capillaries. Angiogenesis. 2013;16:297–307.
Basak S, Das MK, Duttaroy AK. Fatty acid-induced angiogenesis in first trimester placental trophoblast cells: possible roles of cellular fatty acid-binding proteins. Life Sci. 2013;93:755–62.
Bishop-Bailey D. PPARs and angiogenesis. Biochem Soc Trans. 2011;39:1601–5.
Terada S, Goto M, Kato M, Kawanaka K, Shimokawa T, Tabata I. Effects of low-intensity prolonged exercise on PGC-1 mRNA expression in rat epitrochlearis muscle. Biochem Biophys Res Commun. 2002;296:350–4.
Fujimoto E, Yamaguchi W, Terada S, Higuchi M, Tabata I. Change in PGC-1alpha expression in rat skeletal muscle after low-intensity prolonged swimming exercise. J Physiol Anthropol. 2011;30:23–7.
Raney MA, Yee AJ, Todd MK, Turcotte LP. AMPK activation is not critical in the regulation of muscle FA uptake and oxidation during low-intensity muscle contraction. Am J Physiol Endocrinol Metab. 2005;288:E592–8.
Akasaki Y, Miyata M, Eto H, Shirasawa T, Hamada N, Ikeda Y, et al. Repeated thermal therapy up-regulates endothelial nitric oxide synthase and augments angiogenesis in a mouse model of hindlimb ischemia. Circ J. 2006;70:463–70.
Pranskunas A, Pranskuniene Z, Milieskaite E, Daniuseviciute L, Kudreviciene A, Vitkauskiene A, et al. Effects of whole body heat stress on sublingual microcirculation in healthy humans. Eur J Appl Physiol. 2015;115:157–65.
Widanski IB, Richardson D, Bruckner G. Effect of urate on nitric oxide microcirculatory response in the rat tail to body heating. Microcirculation. 2002;9:125–31.
Kivela R, Silvennoinen M, Lehti M, Jalava S, Vihko V, Kainulainen H. Exercise-induced expression of angiogenic growth factors in skeletal muscle and in capillaries of healthy and diabetic mice. Cardiovasc Diabetol. 2008;7:13.
Gute D, Laughlin MH, Amann JF. Regional changes in capillary supply in skeletal muscle of interval-sprint and low-intensity, endurance-trained rats. Microcirculation. 1994;1:183–93.
Yokokawa Y, Hongo M, Urayama H, Nishimura T, Kai I. Effects of low-intensity resistance exercise with vascular occlusion on physical function in healthy elderly people. Biosci Trends. 2008;2:117–23.
Hoier B, Passos M, Bangsbo J, Hellsten Y. Intense intermittent exercise provides weak stimulus for vascular endothelial growth factor secretion and capillary growth in skeletal muscle. Exp Physiol. 2013;98:585–97.
Wallace JP. Exercise in hypertension. A clinical review. Sports Med. 2003;33:585–98.
Goldberg RB. Cytokine and cytokine-like inflammation markers, endothelial dysfunction, and imbalanced coagulation in development of diabetes and its complications. J Clin Endocrinol Metab. 2009;94:3171–82.
Kampoli AM, Tousoulis D, Briasoulis A, Latsios G, Papageorgiou N, Stefanadis C. Potential pathogenic inflammatory mechanisms of endothelial dysfunction induced by type 2 diabetes mellitus. Curr Pharm Des. 2011;17:4147–58.
Persson F, Rossing P, Hovind P, Stehouwer CD, Schalkwijk CG, Tarnow L, et al. Endothelial dysfunction and inflammation predict development of diabetic nephropathy in the Irbesartan in Patients with Type 2 Diabetes and Microalbuminuria (IRMA 2) study. Scand J Clin Lab Invest. 2008;68:731–8.
Patte C, Rothhut B, Russo-Marie F, Blanquet PR. Possible involvement of a lipocortin in the initiation of DNA synthesis by human endothelial cells. Exp Cell Res. 1991;197:12–20.
Xing Z, Gauldie J, Cox G, Baumann H, Jordana M, Lei XF, et al. IL-6 is an antiinflammatory cytokine required for controlling local or systemic acute inflammatory responses. J Clin Invest. 1998;101:311–20.
The authors wish to thank Marcio Marinho Gonzalez for his excellent technical assistance. This study was supported by grants from FAPERJ (Fundação de Amparo à Pesquisa, Rio de Janeiro, Brazil), CNPq (Conselho Nacional de Desenvolvimento Tecnológico) and FIOCRUZ (Fundação Oswaldo Cruz).
The authors do not have any conflicts of interest to declare. The authors declare that there are no competing financial interests in relation to the present study.
R.d.M., M.B.G and E.T. conceived of and designed the study; D.V.B and R.d.M. performed the experiments; R.d.M., M.B.G and E.T. analyzed the data and interpreted the results of the experiments; R.d.M. drafted the manuscript; R.d.M., D.V.B, M.B.G and E.T. edited and revised the manuscript. All authors approved of the final version of the manuscript.
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de Moraes, R., Van Bavel, D., Gomes, M.d.B. et al. Effects of non-supervised low intensity aerobic excise training on the microvascular endothelial function of patients with type 1 diabetes: a non-pharmacological interventional study. BMC Cardiovasc Disord 16, 23 (2016). https://doi.org/10.1186/s12872-016-0191-9
- Exercise training
- Microvascular rarefaction
- Endothelial dysfunction
- Laser Doppler flowmetry