Fig. 1

Obstructive femoral samples from diagnostic images to quantified histomorphometry. A and B are preoperative magnetic resonance images of two male patients that present obstructive level of stenosis of femoral artery (blue arrow) caused by the atherosclerotic lesions C and D respectively. Obstruction in these lesions is demonstrated histologically (arrowhead). Lesion C is dominated by nodular calcification, closely seen in the magnified image (thin arrow) surrounded by fibrin (white asterisk), while lesion D has mainly sheet calcification (black asterisk) in the magnified image. The trained algorithm (E) and (F) recognizes and calculates area proportion of nodular calcification (blue color) and sheet calcification (red color) to the sectioned plaque (green color) area. The calculated area proportion in E is 0.26 for nodular calcification and 0.10 for sheet calcification, while in F is 0.06 for nodular calcification and 0.40 for sheet calcification