Skip to main content

Subacute stent thrombosis with spontaneously resolved secondary thrombi in paroxysmal nocturnal hemoglobinuria: a case report



Stent thrombosis (ST) is a serious complication; however, a method to prevent ST in patients with thrombophilic diseases has not been established.

Case presentation

We report a case of subacute ST in a patient with paroxysmal nocturnal hemoglobinuria (PNH) who was receiving continuous heparin treatment in addition to the usual dual antiplatelet therapy for contrast defects at the proximal site of the occluded right coronary artery and the proximal site of the left circumflex artery. Despite the resolution of thrombi in secondary lesions, subacute ST occurred. After percutaneous coronary intervention for ST, triple therapy, including oral anticoagulation for PNH-related thrombosis, was initiated. The patient subsequently underwent craniotomy hematoma removal for hemorrhagic cerebral infarction.


Reported cases of ST in patients with PNH are very few, and this case adds evidence with respect to antithrombotic therapy in patients with thrombotic tendencies. Both thrombosis and bleeding should be considered when administering antithrombotic therapy to patients with thrombotic diseases. If there are specific treatments for thrombophilic diseases, they should be initiated early.

Peer Review reports


Stent thrombosis (ST) is a fatal complication associated with a mortality rate of 5–45% and occurs in 0.6% of patients with acute coronary syndromes, even with intracoronary imaging techniques [1]. High platelet counts and other factors are reported to be risk factors for ST [2]; however, the association of thrombogenic diseases with ST is unclear. Paroxysmal nocturnal hemoglobinuria (PNH) occurs because of an acquired mutation of the phosphatidylinositol glycan class A (PIGA) gene, and thrombosis should be considered a complication [3]. Reports on ST in PNH are very few. Herein, we report about a patient with PNH who developed ST despite the resolution of thrombi in secondary lesions due to continuous heparin use and conventional dual antiplatelet therapy (DAPT), followed by craniotomy hematoma removal for a hemorrhagic cerebral infarction. This case adds to the evidence for antithrombotic therapy after drug-eluting stent implantation in patients with PNH.

Case presentation

A 73-year-old man with myelodysplastic syndrome for 13 years was diagnosed with PNH 2 years ago based on hemolysis, a negative direct Coombs test, and PNH erythrocyte and granulocyte concentrations of 14.6% and 43.1%, respectively.

The patient presented to the emergency department of our hospital with chest pain, and electrocardiography (ECG) revealed an acute myocardial infarction (AMI) (Fig. 1). Transthoracic echocardiography detected decreased wall motion in the inferior wall region. Emergency coronary angiography (CAG) showed contrast defects at the proximal site of the occluded right coronary artery (RCA) and the proximal site of the left circumflex artery (LCX) (Fig. 2a, b, and c). Aspirin (200 mg) was administered in the emergency department, and clopidogrel 300 mg (Japanese loading dose) was added at the start of percutaneous coronary intervention (PCI). An everolimus-eluting stent (2.75 × 24 mm) was deployed from the distal RCA to the posterior descending artery (Fig. 2d). Heparin was injected to exceed 250 s of activated clotting time (ACT) during PCI, and a sufficiently dilated lumen without malapposition or edge dissection was confirmed using intravascular ultrasound (IVUS). The maintenance dose of DAPT was aspirin 100 mg and clopidogrel 75 mg daily. Continuous heparin was administered to treat thrombi from secondary lesions after PCI, and the heparin dose was gradually increased to 25,000 units/day based on activated partial thromboplastin time (APTT). Two days later, the patient developed chest pain again, and ECG revealed AMI of the inferior wall (Fig. 3); therefore, an emergency CAG was performed. Despite resolution of the defect at the LCX (Fig. 4a), subacute ST occurred in the treated area of the RCA (Fig. 4b). Although the patient had a normal sinus rhythm and was not in shock when the stent thrombosis occurred, intra-aortic balloon pumping (IABP) was implanted to increase coronary blood flow to prevent new thrombus formation and because ST has a high mortality rate, followed by thrombus aspiration, and balloon dilatation were performed during the second PCI (Fig. 4c). As the final IVUS during the initial PCI confirmed that there was no issue with stent implantation, IVUS was not performed during PCI for stent thrombosis. Considering the possibility that the patient maybe a poor metabolizer of clopidogrel, clopidogrel was changed to prasugrel 3.75 mg daily (Japanese dose). However, the normal activity of the clopidogrel metabolizer CYP2C19 was confirmed more than 2 weeks later. Heparin-induced thrombocytopenia (HIT) antibodies were absent, and the change in platelet levels was not suspected to be due to HIT.

Fig. 1
figure 1

Electrocardiogram from the emergency department. Acute myocardial infarction in the right coronary artery territory is suspected

Fig. 2
figure 2

a, b Right anterior oblique (RAO) and left anterior oblique (LAO) caudal views of the left coronary artery (LCA) at the initial acute myocardial infarction (AMI). Contrast defects were detected at the proximal site of the left coronary artery (white arrows). c, d The AMI lesion is in the right coronary artery (RCA). The LAO-cranial view of the RCA before percutaneous coronary intervention (PCI) and after an everolimus-eluting stent (2.75 × 24 mm) was implanted. A contrast defect proximal to the lesion in the right coronary artery is noted (white arrows)

Fig. 3
figure 3

Electrocardiogram of chest pain 2 days after the initial PCI. The right coronary artery appears to be occluded

Fig. 4
figure 4

a RAO-caudal view of the LCA when stent thrombosis (ST) occurred 2 days after the first PCI. The contrast defect observed in the previous coronary angiography had improved. b This is the image of the ST in the LAO-cranial view of the RCA. c The second PCI was performed with intra-aortic balloon pumping, thrombus aspiration, and balloon dilatation. The improvement in blood flow in the occlusion was confirmed in the LAO-cranial view of the RCA

Two days after ST, IABP was discontinued, and continuous heparin was replaced with a direct oral coagulant (apixaban, at a dose of 5 mg twice daily) as a thrombotic treatment for PNH, and cardiac rehabilitation was continued. Although we planned to discontinue triple therapy during hospitalization, the patient was still on aspirin and prasugrel. Ten days after the ST, cranial magnetic resonance imaging was performed because of the development of diplopia. Hemorrhagic cerebral infarction of the left cerebellum in the territory of the posterior inferior cerebellar artery was diagnosed (Fig. 5a). After consultation with a neurologist, antithrombotic therapy was reduced from triple therapy to prasugrel alone, and computed tomography (CT) on the following day showed no obvious hematoma enlargement. However, 13 days after ST (3 days after the diagnosis of hemorrhagic cerebral infarction), the patient developed bradykinesia and underwent craniotomy hematoma removal because of an expanded hematoma observed on CT (Fig. 5b). Eighteen days after ST (5 days after craniotomy hematoma removal), antithrombotic therapy was restarted with aspirin alone because prasugrel had been discontinued owing to hematoma enlargement. The patient was discharged after rehabilitation and introduction of ravulizumab, a long-acting C5 inhibitor, for the treatment of PNH (Fig. 6). Ravulizumab was administered at an initial dose of 2700 mg, followed by 3000 mg 2 weeks later, and 3000 mg every 8 weeks thereafter. Treatment with ravulizumab injection and aspirin monotherapy as an antithrombotic therapy was continued. No evidence of thrombosis, such as new myocardial infarction or cerebral infarction, was noted for more than 1 year.

Fig. 5
figure 5

a T2-weighted magnetic resonance imaging (MRI) performed for diplopia 10 days after the second PCI. A heterogeneous low-signal area in the left cerebellar hemisphere and a high-signal area in its limbus (yellow arrowheads) in the posterior inferior cerebellar artery territory can be observed. Based on the symptoms and images, hemorrhagic cerebral infarction was diagnosed. b Three days after MRI revealed the hemorrhagic cerebral infarction, head computed tomography (CT) was performed because of the occurrence of bradykinesia. CT showed hemorrhage (high density area) and edema around it (low density area) (yellow arrowheads). The hematoma and edema have worsened and compressed the fourth ventricle

Fig. 6
figure 6

Course of treatment (mainly antithrombotic therapy) during hospitalization. Abbreviations: AMI, acute myocardial infarction; ST, stent thrombosis; IABP, intra-aortic balloon pumping

Discussion and conclusions

The incidence of ST has been reduced because of technological advances and DAPT; however, it remains as a fatal complication that cannot be completely prevented [1]. PCI using intracoronary imaging for AMI reduces the risk of ST compared to CAG-guided PCI, although it is reported to cause ST in 0.6% of patients and is fatal in 5–45% of cases [1]. The risk varies according to the time of ST onset. In the acute to subacute phase, this includes ulcerated lesions, baseline and final thrombolysis in myocardial infarction flow of 0 or 1, younger age, diabetes mellitus requiring insulin, history of congestive heart failure, and baseline platelet count [2]. The incidence of ST in patients with diseases with thrombotic tendencies, such as PNH, is unknown.

PNH is a rare clonal hematopoietic stem cell disorder caused by an acquired mutation in the PIGA gene that results in the deletion of the glycosylphosphatidylinositol anchor proteins CD55 and CD59 [3]. Thrombosis is a serious complication in patients with PNH, accounting for approximately 40–67% of deaths with identifiable causes [4]. Multiple factors contribute to thrombus formation in PNH [3]. To the best of our knowledge, no study has reported the incidence of ST in patients with PNH, and case reports are very few.

In this case, the thrombotic tendency of PNH was related to AMI and thrombi in the rest of the coronary arteries. Heparin was adjusted with reference to ACT during PCI, as recommended by the guidelines [5]. Given the anticoagulant therapy for the treatment of secondary thrombi due to PNH [3, 4], continuous heparin was administered after PCI, in addition to the usual DAPT. Heparin was adjusted according to APTT values, and the dose was incrementally increased to 25,000 units/day, although the patient had chest pain and ECG changes again 2 days after PCI. CAG showed that the thrombus in the LCX had disappeared; however, ST occurred. The patient was a normal metabolizer of clopidogrel; therefore, DAPT was as effective as usual, and no antibodies or platelet changes were noted to suspect HIT. The thrombotic tendency of PNH could be related to the development of ST, and a case of repeated ST in a PNH patient with DAPT has been reported [6].When a drug-eluting stent is implanted in a patient with PNH complicated by thrombosis, antithrombotic therapy to eliminate the PNH-induced thrombus may not prevent ST.

The occurrence of thrombosis in the central nervous system of patients with PNH is reported to be 14.0% in the cerebral veins, 6.9% in the cerebral sinuses, and 4.9% in the cerebral arteries [7]. As PNH is associated with ST, anticoagulation was deemed necessary as a treatment for PNH thrombosis. After PCI for ST, the patient was switched to direct oral anticoagulants from continuous heparin, in addition to conventional DAPT. Triple therapy was only planned for use during hospitalization. Hemorrhagic cerebral infarction was identified 10 days after the second PCI. Whether cerebral infarction was catheter-related or a complication of PNH was unclear. While thrombotic complications occur in patients with PNH, hemorrhagic complications due to antithrombotic therapy should also be considered.

Effective treatments for patients with PNH include terminal complement inhibition and allogeneic hematopoietic stem cell transplantation [8]. Eculizumab is a C5 inhibitor that is effective in preventing thrombosis, and ravulizumab is a longer-acting C5 inhibitor than conventional eculizumab, reducing the burden of patient visits [8, 9]. In the present case, ravulizumab was started after the patient had stabilized following the craniotomy hematoma removal, and embolic or bleeding complications did not occur for more than 1 year. PNH clones may resolve spontaneously, and not all patients require treatment [10]. However, C5 inhibitors should be administered to patients with severe thrombosis at the earliest.

PNH is a risk factor for thrombosis, and ST occurs despite the resolution of thrombi in secondary lesions from continuous heparin administration in addition to conventional DAPT. Patients with thrombophilic diseases who undergo PCI may be prone to ST, and decisions regarding the type of antithrombotic therapy should factor the possibility of both thrombosis and bleeding. If specific treatments are available for thrombophilic diseases, they should be initiated without delay.

Availability of data and materials

Data could be obtained upon request to the corresponding author.



Stent thrombosis


Paroxysmal nocturnal hemoglobinuria


Phosphatidylinositol glycan class A


Dual antiplatelet therapy




Acute myocardial infarction


Coronary angiography


Right coronary artery


Left circumflex artery


Percutaneous coronary intervention


Activated clotting time


Intravascular ultrasound


Activated partial thromboplastin time


Intra-aortic balloon pumping


Heparin-induced thrombocytopenia


Computed tomography


Right anterior oblique


Left anterior oblique


Left coronary artery


Magnetic resonance imaging


  1. Ullrich H, Münzel T, Gori T. Coronary stent thrombosis- predictors and prevention. Dtsch Arztebl Int. 2020;117:320–6.

    PubMed  PubMed Central  Google Scholar 

  2. Dangas GD, Caixeta A, Mehran R, Parise H, Lansky AJ, Cristea E, Brodie BR, Witzenbichler B, Guagliumi G, Peruga JZ, Dudek D, Möeckel M, Stone GW. Harmonizing outcomes with revascularization and stents in acute myocardial infarction (HORIZONS-AMI) trial investigators. Frequency and predictors of stent thrombosis after percutaneous coronary intervention in acute myocardial infarction. Circulation. 2011;123:1745–56.

    Article  Google Scholar 

  3. Brodsky RA. Paroxysmal nocturnal hemoglobinuria. Blood. 2014;124:2804–11.

    CAS  Article  Google Scholar 

  4. Hill A, Kelly RJ, Hillmen P. Thrombosis in paroxysmal nocturnal hemoglobinuria. Blood. 2013;121:4985–96.

    CAS  Article  Google Scholar 

  5. O’Gara PT, Kushner FG, Ascheim DD, Casey DE Jr, Chung MK, de Lemos JA, Ettinger SM, Fang JC, Fesmire FM, Franklin BA, Granger CB, Krumholz HM, Linderbaum JA, Morrow DA, Newby LK, Ornato JP, Ou N, Radford MJ, Tamis-Holland JE, Tommaso CL, Tracy CM, Woo YJ, Zhao DX. 2013 ACCF/AHA guideline for the management of ST-elevation myocardial infarction: a report of the American college of cardiology foundation/american heart association task force on practice guidelines. J Am Coll Cardiol. 2013;61:e78-140.

    Article  Google Scholar 

  6. De Maria GL, Sarwar R, Banning AP. Eculizumab treatment for paroxysmal nocturnal haemoglobinuria in a patient with recurrent simultaneous multivessel coronary stent thrombosis. Oxf Med Case Reports. 2015;2015:167–9.

    Article  Google Scholar 

  7. Ziakas PD, Poulou LS, Rokas GI, Bartzoudis D, Voulgarelis M. Thrombosis in paroxysmal nocturnal hemoglobinuria: sites, risks, outcome. An overview J Thromb Haemost. 2007;5:642–5.

    CAS  Article  Google Scholar 

  8. Devos T, Meers S, Boeckx N, Gothot A, Deeren D, Chatelain B, Chatelain C, Devalet B. Diagnosis and management of PNH: review and recommendations from a Belgian expert panel. Eur J Haematol. 2018;101:737–49.

    Article  Google Scholar 

  9. Kulasekararaj AG, Hill A, Rottinghaus ST, Langemeijer S, Richard Wells F, Gonzalez-Fernandez A, Gaya A, Lee JW, Gutierrez EO, Piatek CI, Szer J, Risitano A, Nakao S, Bachman E, Shafner L, Damokosh AI, Ortiz S, Röth A, Latour R. Ravulizumab (ALXN1210) vs eculizumab in C5-inhibitor–experienced adult patients with PNH: the 302 study. Blood. 2019;133(6):540–9.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  10. Hillmen P, Lewis SM, Bessler M, Luzzatto L, Dacie JV. Natural history of paroxysmal nocturnal hemoglobinuria. N Engl J Med. 1995;333:1253–8.

    CAS  Article  Google Scholar 

Download references


Not applicable.


Not applicable.

Author information

Authors and Affiliations



HK have wrote the paper. NW, AE, YH have revised the manuscript critically and KT has approved the manuscript finally. All authors have read the manuscript and approved the submission.

Corresponding author

Correspondence to Hiroshi Kawahara.

Ethics declarations

Ethics approval and consent to participate

This study was approved by the ethics committee of Shimane University Faculty of Medicine (No. 5327).

Consent for publication

Written informed consent was obtained from the patient for the publication of this study.

Competing interests

Not applicable.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Kawahara, H., Watanabe, N., Endo, A. et al. Subacute stent thrombosis with spontaneously resolved secondary thrombi in paroxysmal nocturnal hemoglobinuria: a case report. BMC Cardiovasc Disord 22, 408 (2022).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI:


  • Paroxysmal nocturnal hemoglobinuria
  • Percutaneous coronary intervention
  • Stent thrombosis
  • Antithrombotic therapy
  • Case report