Fig. 5

IL-1β and IL1R1 showed a higher expression level in the RHD patient mitral valve compared with the CHD patient. a-c IL-1β and IL1R1 in the mitral valve were assayed by IHC as described in the Methods using IL-1β or IL1R1 antibody b and c. Normal rabbit IgG was used to substitute antibodies as a negative control (a). The graph is representative of 6 CHD patients and 6 RHD patients. Bar = 50 μm. d The expression of IL-1β and IL1R1 was assessed by Western blot as described in the Methods section. β-actin was used as an internal control