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Prevalence of peripheral arterial disease in patients at non-high cardiovascular risk. Rationale and design of the PANDORA study

BMC Cardiovascular Disorders volume 10, Article number: 35 (2010) Cite this article

Abstract

Background

Lower extremity peripheral arterial disease (PAD) is a marker of widespread atherosclerosis. Individuals with PAD, most of whom do not show typical PAD symptoms ('asymptomatic' patients), are at increased risk of cardiovascular ischaemic events. American College of Cardiology/American Heart Association guidelines recommend that individuals with asymptomatic lower extremity PAD should be identified by measurement of ankle-brachial index (ABI). However, despite its associated risk, PAD remains under-recognised by clinicians and the general population and office-based ABI detection is still poorly-known and under-used in clinical practice. The Prevalence of peripheral Arterial disease in patients with a non-high cardiovascular disease risk, with No overt vascular Diseases nOR diAbetes mellitus (PANDORA) study has a primary aim of assessing the prevalence of lower extremity PAD through ABI measurement, in patients at non-high cardiovascular risk, with no overt cardiovascular diseases (including symptomatic PAD), or diabetes mellitus. Secondary objectives include documenting the prevalence and treatment of cardiovascular risk factors and the characteristics of both patients and physicians as possible determinants for PAD under-diagnosis.

Methods/Design

PANDORA is a non-interventional, cross-sectional, pan-European study. It includes approximately 1,000 primary care participating sites, across six European countries (Belgium, France, Greece, Italy, The Netherlands, Switzerland). Investigator and patient questionnaires will be used to collect both right and left ABI values at rest, presence of cardiovascular disease risk factors, current pharmacological treatment, and determinants for PAD under-diagnosis.

Discussion

The PANDORA study will provide important data to estimate the prevalence of asymptomatic PAD in a population otherwise classified at low or intermediate risk on the basis of current risk scores in a primary care setting.

Trial registration number

Clinical Trials.gov Identifier: NCT00689377.

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Background

Lower extremity peripheral arterial disease (PAD) is considered to be a marker of widespread atherosclerosis. In patients without overt cardiovascular disease (CVD), the presence of PAD predicts approximately a 30% risk of myocardial infarction, ischaemic stroke, and vascular death over 5 years. Patients with PAD show a 2- to 6-fold increase in death from cardiovascular causes and a greater risk of limb amputation than those without PAD. Individuals with PAD also experience higher 1-year rates of cardiovascular death (2.5%) and major cardiovascular events (21.1%) caused by an increase in atherothrombotic events, compared with patients with coronary artery disease (1.9% and 15.2%, respectively) or with any cerebrovascular disease (2.1% and 14.5%, respectively) [1–7].

Despite its association with severe health risk, PAD remains under-recognised by clinicians and the general population [8, 9]. This may be due to the fact that the majority of individuals with lower extremity PAD do not experience recognisable ischaemic symptoms in the limb and are considered 'asymptomatic'. Nevertheless, these individuals share the unfavourable cardiovascular prognosis of symptomatic patients, as they show a risk profile comparable to that of patients with symptomatic lower extremity PAD or with coronary heart disease (CHD) [1, 2, 10, 11]. Patients diagnosed with PAD, including those with asymptomatic PAD, should be treated to reach the recommended therapeutic goals, including low-density lipoprotein cholesterol (LDL-C) target levels <2.6 mmol/L (100 mg/dL) [12], which are similar to those recommended for high risk patients, i.e. those with CHD or CHD risk equivalents - although more challenging than that recommended for individuals at moderate CVD risk, i.e. 3.3 mmol/L (130 mg/dL) [2, 12, 13].

Ankle-brachial index (ABI) value ≤0.90 is the recognised cut-off for diagnosis of PAD. According to a few observational studies, pathological ABI values are frequently observed among elderly patients (aged 70+ years or 50-69 years but with a history of cigarette smoking) who are otherwise not classified as high risk [1, 2]. Indeed, the prevalence of a pathological ABI still remains substantially unknown among patients classified at intermediate or low risk according to the current risk scores. The American College of Cardiology (ACC)/American Heart Association (AHA) guidelines recommend screening for both symptomatic and asymptomatic PAD, by physical examination and/or ABI measurement, in order to identify patients with asymptomatic lower limb PAD and to offer appropriate therapeutic interventions that diminish risk of myocardial infarction, stroke, and death.

Hence, the Prevalence of peripheral Arterial disease in patients with a non-high CVD risk, with No overt vascular Diseases nOR diAbetes mellitus (PANDORA) study was designed to provide updated information on the prevalence of PAD, in the context of traditional risk factor assessment, and insight on clinical characteristics as possible determinants for PAD under-diagnosis.

Methods/Design

PANDORA is a non-interventional, cross-sectional, pan-European study of patients with a non-high CVD risk, defined as having at least two CVD risk factors according to National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III [ATP III]) guidelines, with no overt CVDs or diabetes mellitus. Patients with definite symptoms of PAD will be also excluded (ClinicalTrials.gov Identifier: NCT00689377).

Approximately 1,000 sites will participate in the study in six European countries (Belgium, France, Greece, Italy, The Netherlands, Switzerland). Study objectives and selection criteria are listed in Table 1. Since the majority of individuals with a CVD risk factor score of 0 or 1 have a low 10-year risk, i.e. below 10%, these patients are excluded from the study population [13]. Study variables, as listed in Table 2, are collected in the Subject Record Form. Data are captured electronically in most participating countries.

Table 1 Study objectives and selection criteria
Full size table
Table 2 Data captured in the Patient Record Form (PRF)
Full size table

Prior to the inclusion of the first patient, investigators are requested to complete an Investigator Questionnaire regarding their personal experience and perception of the management of CVD risk, and attitude towards CVD diagnosis, guidelines and goals. The study will collect data on knowledge and behaviour in CVD diagnosis and management, quantity of CVD cases experienced, and attitudinal statements about guidelines and goals.

Patients recruited will be asked to complete a Patient Questionnaire which aims to collect data on awareness of CVD risk factors, knowledge and awareness of PAD, CVD risk perception and treatment, frequency of medical appointments.

The study conforms to the ethical principles outlined in the Declaration of Helsinki and is consistent with ICH/Good Clinical Practice, applicable regulatory requirements, and the AstraZeneca policy on Bioethics.

ABI measurement

Each investigator receives theoretical and practical training on the ABI measurement technique according to ACC/AHA guidelines for the management of patients with PAD [1, 2]. Right and left ABI measurement is performed at rest by measuring the systolic blood pressure from both brachial arteries and from both the dorsalis pedis and posterior tibial arteries after the patient has been at rest in the supine position for 10 minutes. Measurements are taken with blood pressure cuffs that are appropriately sized to the patient's lower calf (immediately above the ankle). The hand-held Doppler ultrasonography instrument Elite 100R, Nicolet Vascular Inc. (Golden, Colorado, USA), with an 8 MHz vascular probe, is used to measure systolic pressures and ABI. The Elite 100R vascular Doppler device was chosen since it has been used in major published studies on PAD detection [1, 14]. The 8 MHz probe is preferred over the 5 MHz probe, due to its higher sensitivity and reliability in detecting both superficial and deep arteries.

For ABI calculations, the higher of the two brachial artery systolic pressures, and the higher of the dorsalis pedis and posterior tibial pressures in each ankle, are selected. The right and left ABI values are separately calculated by dividing the higher of the two ankle systolic pressures in that leg by the higher brachial artery systolic pressure. Calculated ABI values are recorded to two decimal places. Each patient is considered to have PAD if ABI in either leg is 0.90 or less [1, 2, 15].

Statistical analysis

The statistical analyses will be descriptive in nature. Data will be presented as mean ± standard deviation or median with minimum and maximum. Summary data will be presented from all available data. Categorical data will be described by the number and percentage of patients in each category. Missing observations will be presented in tables as a separate category. The calculation of proportions will not include the missing category.

The primary objective of this survey is to determine the proportion of patients with pathological ABI, according to ACC/AHA guidelines for the management of patients with PAD. The overall sample size calculations were based on a confidence interval approach to ensure that the estimates will have adequate precision. A reasonable requirement on the precision is that the incidence should be determined within ± 1%, i.e. the length of the two-sided 99% confidence should not exceed 1 percentage value in each direction from the estimated value. Based on these calculations and expecting a 15% prevalence of individuals with pathological ABI in the selected population [14], the minimum sample size considered sufficient to meet the objectives of this protocol is 8,454 patients. However, to account for potential drop-outs, the survey plans to enrol 9,000 patients. Such sample size also ensures that the proportion reporting on the primary endpoint can be estimated with sufficient precision, on a 'by-country' basis, to represent the country-based heterogeneity of this population.

Results and discussion

PAD, especially in the asymptomatic stage is common, and its prompt screening and treatment can reduce cardiovascular risk [2].

Although PAD shares the same risk factors of other CVDs and the guidelines recommend the same preventive treatments and therapeutic targets, there is low awareness of PAD (26% for PAD, compared with 74% for stroke, and 67% for coronary artery disease and heart failure, respectively) [8]. There appears to be greater awareness even of a rare disease such as Lou Gehrig's disease (36%), or conditions such as multiple sclerosis (42%) and cystic fibrosis (29%) [12].

The detection of asymptomatic people with an ABI ≤0.90 allows for the identification of a subgroup at higher cardiovascular risk than can be determined based on the usual CVD risk assessment. Diagnosis of asymptomatic PAD in such a population assigns these individuals to the 'secondary prevention risk level' [12].

ABI measurement is now accepted as a simple and reliable vascular test to assess leg perfusion. ABI testing is appropriate in populations targeted from the epidemiological database to be at risk for PAD. Among patients with no history of CVD or diabetes, ABI measurement is recommended in certain categories appearing to be at moderate CVD risk, including individuals at higher-risk ages, and with risk factors typically associated with PAD, such as cigarette smoking [2]. Quite recently, Cacoub et al. assessed the prevalence of PAD in a population of patients with two or more cardiovascular risk factors [16]. Diehm et al. provided data on the prevalence of PAD in a large population of unselected individuals aged ≥65 years from the primary care setting [17]. However, data on the prevalence of asymptomatic PAD in a large population otherwise classified at low or intermediate risk are lacking.

Office-based detection of ABI is the most cost-effective tool for lower extremity PAD detection and provides objective data both for the diagnosis in epidemiological surveys and in-office practice, and monitoring of efficacy of therapeutic interventions [2, 18, 19].

ACC/AHA guidelines state that the initial responsibility for the detection of lower extremity PAD should be with primary-care providers, because such providers are best positioned to determine an at-risk population and to initiate educational, lifestyle, and cardiovascular risk reduction therapies [2].

Conclusions

Low awareness of PAD and scarce familiarity with the ABI technique seems to greatly undermine the diagnosis of asymptomatic PAD patients, who are at comparable risk to patients with CHD and should be appropriately treated. Unfortunately, most asymptomatic patients do not receive a diagnosis of PAD prior to the occurrence of a morbid or fatal ischaemic disease or event [1, 2]. This is particularly true for individuals with asymptomatic PAD otherwise classified at low or intermediate risk on the basis of current risk scores. Information on the prevalence of asymptomatic PAD in such a setting is lacking. Improving PAD awareness among clinicians and the public is crucial for effective CVD prevention, early detection and integrated treatment. The PANDORA study will provide important pan-European data to estimate the prevalence of asymptomatic PAD in people with a non-high CVD risk in a primary care setting, by using the ABI technique, a risk-free gold-standard diagnostic test for PAD. This study may provide additional insights on identifying an at-risk segment of population that may benefit from appropriate preventive therapy.

Abbreviations

ABI:

ankle-brachial index

ATC:

anatomical therapeutic chemical

CHD:

coronary heart disease

CVD:

cardiovascular disease

HDL:

high-density lipoprotein

LDL:

low-density lipoprotein

PAD:

peripheral arterial disease

PANDORA:

Prevalence of peripheral Arterial disease in patients with a non-high CVD risk, with No overt vascular Diseases nOR diAbetes mellitus

PCI:

percutaneous coronary intervention.

References

  1. Hirsch AT, Criqui MH, Treat-Jacobson D, Regensteiner JG, Creager MA, Olin JW, Krook SH, Hunninghake DB, Comerota AJ, Walsh ME, McDermott MM, Hiatt WR: Peripheral arterial disease detection, awareness, and treatment in primary care. JAMA. 2001, 286: 1317-1324. 10.1001/jama.286.11.1317.

    Article  CAS  PubMed  Google Scholar 

  2. Hirsch AT, Haskal ZJ, Hertzer NR, Bakal CW, Creager MA, Halperin JL, Hiratzka LF, Murphy WR, Olin JW, Puschett JB, Rosenfield KA, Sacks D, Stanley JC, Taylor LM, White CJ, White J, White RA, Antman EM, Smith SC, Adams CD, Anderson JL, Faxon DP, Fuster V, Gibbons RJ, Halperin JL, Hiratzka LF, Hunt SA, Jacobs AK, Nishimura R, Ornato JP, Page RL, Riegel B: ACC/AHA 2005 guidelines for the management of patients with peripheral arterial disease (lower extremity, renal, mesenteric, and abdominal aortic): executive summary a collaborative report from the American Association for Vascular Surgery/Society for Vascular Surgery, Society for Cardiovascular Angiography and Interventions, Society for Vascular Medicine and Biology, Society of Interventional Radiology, and the ACC/AHA Task Force on Practice Guidelines (Writing Committee to Develop Guidelines for the Management of Patients With Peripheral Arterial Disease) endorsed by the American Association of Cardiovascular and Pulmonary Rehabilitation; National Heart, Lung, and Blood Institute; Society for Vascular Nursing; TransAtlantic Inter-Society Consensus; and Vascular Disease Foundation. J Am Coll Cardiol. 2006, 47: 1239-1312. 10.1016/j.jacc.2005.10.009.

    Article  PubMed  Google Scholar 

  3. Sans S, Kesteloot H, Kromhout D, on behalf of the Task Force: The burden of cardiovascular diseases mortality in Europe. Task Force of the European Society of Cardiology on Cardiovascular Mortality and Morbidity Statistics in Europe. Eur Heart J. 1997, 18: 1231-1248.

    Article  Google Scholar 

  4. British Heart Foundation: European Cardiovascular Disease Statistics. London. 2005

    Google Scholar 

  5. Murabito JM, D'Agostino RB, Silbershatz H, Wilson WF: Intermittent claudication. A risk profile from The Framingham Heart Study. Circulation. 1997, 96: 44-49.

    Article  CAS  PubMed  Google Scholar 

  6. Treat-Jacobson D, Halverson SL, Ratchford A, Regensteiner JG, Lindquist R, Hirsch AT: A patient-derived perspective of health-related quality of life with peripheral arterial disease. J Nursing Scholarsh. 2002, 34: 55-60. 10.1111/j.1547-5069.2002.00055.x.

    Article  Google Scholar 

  7. Steg PG, Bhatt DL, Wilson PWF, D'Agostino R, Ohman EM, Röther J, Liau CS, Hirsch AT, Mas JL, Ikeda Y, Pencina MJ, Goto S, REACH Registry Investigators: One-year cardiovascular event rates in outpatients with atherothrombosis. JAMA. 2007, 297: 1197-1206. 10.1001/jama.297.11.1197.

    Article  CAS  PubMed  Google Scholar 

  8. Hirsch AT, Murphy TP, Lovell MB, Twillman G, Treat-Jacobson D, Harwood EM, Mohler ER, Creager MA, Hobson RW, Robertson RM, Howard WJ, Schroeder P, Criqui MH, Peripheral Arterial Disease Coalition: Gaps in public knowledge of peripheral arterial disease the first national PAD public awareness survey. Circulation. 2007, 116: 2086-2094. 10.1161/CIRCULATIONAHA.107.725101.

    Article  PubMed  Google Scholar 

  9. Tzou WS, Mohler ER III: Peripheral arterial disease: diagnosis and medical management. Hosp Physician. 2006, 42: 17-25.

    Google Scholar 

  10. Hooi JD, Stoffers HE, Kester AD, Rinkens PE, Kaiser V, van Ree JW, Knottnerus JA: Risk factors and cardiovascular diseases associated with asymptomatic peripheral arterial occlusive disease. The Limburg PAOD Study. Peripheral arterial occlusive disease. Scand J Prim Health Care. 1998, 16: 177-182. 10.1080/028134398750003142.

    Article  CAS  PubMed  Google Scholar 

  11. Hooi JD, Kester AD, Stoffers HE, Overdijk MM, van Ree JW, Knottnerus JA: Incidence of and risk factors for asymptomatic peripheral arterial occlusive disease: a longitudinal study. Am J Epidemiol. 2001, 153: 666-672. 10.1093/aje/153.7.666.

    Article  CAS  PubMed  Google Scholar 

  12. National Cholesterol Education Program (NCEP) Expert panel on detection, evaluation and, treatment of high blood cholesterol in adults (Adult Treatment Panel III): Third report of the National Cholesterol Education Program (NCEP) expert panel on detection, evaluation and, treatment of high blood cholesterol in adults (Adult Treatment Panel III): final report. Circulation. 2002, 106: 3143-3121.

    Google Scholar 

  13. Grundy SM, Cleeman JI, Merz CN, Brewer HB, Clark LT, Hunninghake DB, Pasternak RC, Smith SC, Stone NJ, National Heart, Lung, and Blood Institute; American College of Cardiology Foundation; American Heart Association: Implications of recent clinical trials for the National Cholesterol Education Program Adult Treatment Panel III guidelines. Circulation. 2004, 110: 227-239. 10.1161/01.CIR.0000133317.49796.0E.

    Article  PubMed  Google Scholar 

  14. Fowkes FGR, Low LP, Tuta S, Kozak J, AGATHA Investigators: Ankle-brachial index and extent of atherothrombosis in 8891 patients with or at risk of vascular disease: results of the international AGATHA study. Eur Heart J. 2006, 27: 1861-1867. 10.1093/eurheartj/ehl114.

    Article  PubMed  Google Scholar 

  15. Hiatt WR: Medical treatment of peripheral arterial disease and claudication. New Engl J Med. 2001, 344: 1608-1621. 10.1056/NEJM200105243442108.

    Article  CAS  PubMed  Google Scholar 

  16. Cacoub P, Cambou JP, Kownator S, Belliard JP, Beregi JP, Branchereau A, Carpentier P, Léger P, Luizy F, Maïza D, Mihci E, Herrmann MA, Priollet P: Prevalence of peripheral arterial disease in high-risk patients using ankle-brachial index in general practice: a cross-sectional study. Int J Clin Pract. 2009, 63: 63-70. 10.1111/j.1742-1241.2008.01953.x.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Diehm C, Lange S, Darius H, Pittrow D, von Stritzky B, Tepohl G, Haberl RL, Allenberg JR, Dasch B, Trampisch HJ: Association of low ankle brachial index with high mortality in primary care. Eur Heart J. 2006, 27: 1743-1749. 10.1093/eurheartj/ehl092.

    Article  PubMed  Google Scholar 

  18. Kannel WB: The demographics of claudication and the aging of the American population. Vasc Med. 1996, 1: 60-64.

    CAS  PubMed  Google Scholar 

  19. Greenland P, Abrams J, Aurigemma GP, Bond MG, Clark LT, Criqui MH, Crouse JR, Friedman L, Fuster V, Herrington DM, Kuller LH, Ridker PM, Roberts WC, Stanford W, Stone N, Swan HJ, Taubert KA, Wexler L: Prevention Conference V. Beyond secondary prevention: identifying the high-risk patient for primary prevention: noninvasive tests of atherosclerotic burden: Writing Group III. Circulation. 2000, 101: e16-e22.

    Article  CAS  PubMed  Google Scholar 

Pre-publication history

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Acknowledgements

This study is sponsored by AstraZeneca.

The authors would like to thank Catherine Bryant (QXV Communications, Macclesfield, UK) for her editorial assistance in the manuscript preparation that was funded by AstraZeneca and thank Dr Milena Fioravanti and Dr Barbara Merati (AstraZeneca SpA) for assistance with manuscript preparation and review.

Author information

Authors and Affiliations

  1. Department of Medicine, Vimercate Hospital, via Cesare Battisti 23, Vimercate, (MI), 20059, Italy

    Claudio Cimminiello

  2. Internal Medicine Unit, University Hospital Sant'Orsola-Malpighi, Bologna, Italy

    Claudio Borghi

  3. Clinique Ambroise Paré, Cardiology Department, Thionville, France

    Serge Kownator

  4. Department of Vascular Diseases, Hôpital Erasme, Brussels, Belgium

    Jean Claude Wautrecht

  5. Internal Medicine Department, Blue Cross Hospital, Athens, Greece

    Christos P Carvounis

  6. TweeSteden Hospital, Dr Deelenlaan 5, 5042, AD, Tilburg, The Netherlands

    Stefanus E Kranendonk

  7. General Practitioner, Dufourstrasse 77, 8008, Zurich, Switzerland

    Beat Kindler

  8. R&D Department, AstraZeneca SpA, Milan, Italy

    Mario Mangrella

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  1. Claudio Cimminiello
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the PANDORA Study Investigators

Corresponding author

Correspondence to Claudio Cimminiello.

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Competing interests

CC has no financial or non-financial competing interests. He has acted as a speaker and chairman at scientific meetings sponsored by AstraZeneca, Sanofi-Aventis and Bristol-Myers Squibb.

CB has acted as a consultant or speaker on occasions for Recordati, AstraZeneca, Merck, MSD, Novartis, Boehringer Ingelheim, Takeda, and Schering-Plough, and has received research funding from Boehringer Ingelheim, Sanofi-Aventis, Takeda, Italian Society of Hypertension, and the Regional Drug Agency. He holds shares in Abbott and Bristol-Myers Squibb and has received honoraria as a speaker in international and national meetings. He does not have any conflict of interest relating to the present activity.

SK has been a consultant or speaker for AstraZeneca, Boehringer-Ingelheim, Bristol-Myers Squibb, Daichi-Sankyo, MSD, Novartis, Pfizer, Sanofi-Aventis, Schering-Plough, and Solvay.

JCW has no financial or non-financial competing interests.

CPC has received honoraria from Mount Sinai Medical Center and has received research grants as National Coordinator of five Phase IV local studies. He has received grants and acted as a consultant for AstraZeneca.

SEK has acted as occasional speaker and has received honoraria as National Coordinator of the PANDORA study, and as consultant on PAD Advisory Board

BK has participated as investigator in an AstraZeneca sponsored clinical trial and has received honoraria

MM is an employee of AstraZeneca.

Authors' contributions

CC participated in the study design, evaluated the statistical analysis, and drafted the manuscript. CB participated in the study design, evaluated the statistical analysis, and drafted the manuscript. SK participated in the study design and drafted the manuscript. JCW participated in the study design and drafted the manuscript. CPC participated in the study design and drafted the manuscript. SEK participated in the study design and drafted the manuscript. BK drafted, read and approved the final manuscript. MM participated in the study design, evaluated the statistical analysis, and drafted the manuscript. All authors read and approved the final manuscript.

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Cimminiello, C., Borghi, C., Kownator, S. et al. Prevalence of peripheral arterial disease in patients at non-high cardiovascular risk. Rationale and design of the PANDORA study. BMC Cardiovasc Disord 10, 35 (2010). https://doi.org/10.1186/1471-2261-10-35

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Keywords

  • Peripheral Arterial Disease
  • Peripheral Arterial Disease Patient
  • Coronary Heart Disease Risk Equivalent
  • Overt Cardiovascular Disease
  • Asymptomatic Peripheral Arterial Disease

BMC Cardiovascular Disorders

ISSN: 1471-2261

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