Fig. 5
The effects of zr17-2 on cardiac apoptosis in hydrogen peroxide (H2O2)-stimulated H9C2 cells in vitro. A Western blot analysis of CIRP expression in response to zr17-2 treatment at different times. B Western blot analysis of Nrf2, HO-1, and NQO-1 in response to zr17-2 treatment at different times. C Western blot analysis of apoptosis-associated markers such as Bax, Bcl2, and cleaved-caspase 3 from H9C2 cells in response to H2O2 stimulation in the presence or absence of zr17-2 pretreatment. D Cell survival analysis of H9C2 cells by CCK8 assay in response to H2O2 stimulation in the presence or absence of zr17-2 pretreatment. E The effects of siRNA targeting the CIRP gene on the protein level of CIRP in vitro in the presence or absence of zr17-2. F Cell survival analysis of CIRP-deficient cells by CCK8 assay in response to H2O2 stimulation in the presence or absence of zr17-2 pretreatment. n ≥ 3 in each group. *p < 0.05, **p < 0.001 and ****p < 0.0001