Fig. 3
The effects of zr17-2 pretreatment on cardiac inflammation in MI rats. A Representative images of heart tissue stained with Sirius red or CD68 fluorescent antibody (green) and DAPI (blue) from the MI rats treated with zr17-2 or Saline. B Quantitative analysis of CD68 staining in the peri-infarction areas of the hearts. C-F The mRNA levels of inflammatory factors in the heart tissues such as IL-1β, VCAM-1, ICAM-1, and IL-6. G The serum level of IL-6. H-I Western blot analysis of the protein levels of IL-1β, ICAM-1, and VCAM-1 in the heart. β-actin was used as an internal control. IL-1β, interleukin 1 beta; ICAM-1, intercellular adhesion molecule 1; VCAM-1, vascular cell adhesion molecule 1; n = 3–6 in each group. ** p < 0.01, ***p < 0.001 and **** p < 0.0001