Fig. 7
Validation of the expression of hub genes. A The results of HE: Sham group myocardial cells were neatly arranged in striated patterns.MIRI group exhibited disorganized myocardial cell arrangement, with instances of myocardial rupture, nuclear deformation. B The results of TTC: The myocardial tissue in Sham group typically shows a uniform red coloration. MIRI group myocardial tissue appear pale or white. These areas represent necrotic or infarcted tissue. C, D Western blot analysis of HIF-1α, BNIP3, LC3I and LC3II protein levels in Sham and MIRI group (The experiments were repeated three times). E, F Immunofluorescence analysis of HIF-1α and BNIP3 in Sham and MIRI group. G QPCR results for mRNA levels of hub genes HIF-1α and BNIP3; ***P < 0.001, ****P < 0.0001