Fig. 7

Knockdown of JUP activates the Akt/β-catenin signaling and aggravates cardiac impairment. Mice were administrated with lentiviral vectors-carried OE-USP2 and the additional KD-JUP or KD-NC, followed by ISO injection. A, HW/BW of mice. B, Myocardial injury of mice analyzed by HE staining. C, LVEF and LVFS or mice analyzed by echocardiographic assessment. D, Serum levels of CK, CK-MB, LDH, and cTnT in mice determined using ELISA kits. E, ROS content in the mouse heart determined using DHE staining. F, SOD activity and MDA content in the mouse heart tissue determined using ELISA kits. G, Concentrations of ANP and BNP in the mouse heart tissue determined using ELISA kits. H, Cell apoptosis in the mouse heart tissue examined using TUNEL assay. I, phosphorylation of Akt and protein level of β-catenin in mouse heart tissue examined by WB analysis. In each group, n = 10. Differences were analyzed using the unpaired t test or the two-way ANOVA. *p < 0.05. See full-length blot images in Supplementary Figure S12