Fig. 6

USP2 stabilizes JUP protein to block the Akt/β-catenin signaling cascade. A, Possible substrates of USP2 predicted in the UbiBrowser system. B, JUP protein level in mouse heart tissues examined by WB analysis. See full-length blot images in Supplementary Figure S4. C, protein levels of USP2 and JUP in mouse cardiomyocytes after OE-USP2 transfection determined by WB analysis. See full-length blot images in Supplementary Figure S5. D, ubiquitination of JUP mediated by USP2 examined by ubiquitination and co-IP assays. Supplementary Figure S6-S7. E, JUP protein degradation rates in mouse cardiomyocytes under CHX treatment. Supplementary Figure S8-S9. F, phosphorylation of Akt and protein level of β-catenin in the mouse cardiomyocytes determined by WB analysis. Supplementary Figure S10. G, phosphorylation of Akt and protein level of β-catenin in mouse heart tissue examined by WB analysis. Supplementary Figure S11. For animal experiments, n = 10 in each group. For cellular experiments, three biological replicates were conducted. Differences were analyzed by the unpaired t test or the two-way ANOVA. *p < 0.05