Fig. 4

Mycn activates USP2 transcription. A, USP2 predicted as a target of Mycn predicted in the hTFtarget system and the putative binding sites. B-C, mRNA (B) and protein (C) expression of USP2 in the heart tissue of mice after HCM modeling and OE-Mycn treatment examined by RT-qPCR and WB analysis, respectively. D, binding relationship between Mycn and the USP2 promoter in mouse cardiomyocytes determined by the ChIP assay. E, transcription activity of USP2 promoter in mouse cardiomyocytes transfected with OE-Mycn examined by the luciferase reporter gene assay. For animal experiments, n = 10 in each group. For cellular experiments, three biological replicates were conducted. Differences were analyzed by the unpaired t test or the one-way ANOVA. *p < 0.05. See full-length blot images in Supplementary Figure S3