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Fig. 3 | BMC Cardiovascular Disorders

Fig. 3

From: Mycn ameliorates cardiac hypertrophy-induced heart failure in mice by mediating the USP2/JUP/Akt/β-catenin cascade

Fig. 3

Mycn possesses cardiomyocyte-protective effects in vitro. A, mRNA expression of Mycn, ANP, BNP, and β-MHC in cardiomyocytes after OE-Mycn/OE-NC transfection and ISO treatment examined by RT-qPCR; B, Apoptosis of cardiomyocytes examined by TUNEL assay; C, Protein levels of c-CAS-3, Bcl-2, Bax, IL-1β, TNF-α, and MMP-9 in cardiomyocytes determined using WB analysis; D, Viability of cardiomyocytes examined using MTT assay; E, LDH release in cardiomyocytes analyzed using ELISA. Three biological replicates were performed. Differences were analyzed using the one-way or two-way ANOVA. *p < 0.05. See full-length blot images in Supplementary Figure S1-S2

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BMC Cardiovascular Disorders

ISSN: 1471-2261

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