Fig. 8

Animal experimental validation of the influence of lncRNA KCNQ1OT1 on MI/RI via the miR-377-3p/HMOX1 pathway (n = 5). A: Apoptosis was measured by a TUNEL assay (scale bar = 100 μm). B-D: RT‒qPCR was used to measure lncRNA KCNQ1OT1, miR-377-3p, and HMOX1 expression. E: An ROS assay kit was utilized to measure ROS levels. F and G: Oxidative stress marker kits were used to evaluate SOD and MDA expression. H: An iron analysis kit was used to detect Fe²⁺ changes. I: Western blot analysis was used to detect the levels of CK, LDH, Bax, and Bcl-2. Compared with the control group, ^* P < 0.05, ^** P < 0.01 and ^*** P < 0.001; compared with the MI/RI group, ^# P < 0.05, ^## P < 0.01 and ^### P < 0.001; compared with the MI/RI + si-HMOX1 group, ^P < 0.05, ^^P < 0.01 and ^^^P < 0.001; compared with the MI/RI + si-HMOX1 + miR-377-3p inhibitor group, ^&P < 0.05, ^&& P < 0.01 and ^&&& P < 0.001