Fig. 7
LncRNA KCNQ1OT1 targets miR-377-3p to mediate I/R injury via the HMOX1 pathway (n = 3). A: CCK-8 assay was used to detect the proliferation of HACMs. B: Flow cytometry was used to detect HACMs apoptosis. C-E: RT‒qPCR was used to detect the expression of lncRNA KCNQ1OT1, miR-377-3p, and HMOX1. F: Flow cytometry was used to measure ROS levels. G: MitoSOX Red probe was used to measure mitochondrial ROS levels (scale bar = 100 μm). H-I: Oxidative stress marker kits were utilized to measure SOD and MDA expression. J: An iron analysis kit was used to detect changes in Fe2+. K: Western blot analysis was used to detect the levels of CK, LDH, Bax, and Bcl-2. Compared with the NC group, * P < 0.05, ** P < 0.01 and *** P < 0.001; compared with the H/R group, # P < 0.05, ## P < 0.01 and ### P < 0.001; compared with the H/R + si-HMOX1 group, ^P < 0.05, ^^P < 0.01 and ^^^P < 0.001; compared with the H/R + si-HMOX1 + miR-377-3p inhibitor group, &P < 0.05, && P < 0.01 and &&& P < 0.001