Fig. 6

Inhibition of HMOX1 attenuates miR-377-3p downregulation-induced myocardial injury (n = 3). A: CCK-8 assay was used to detect cell viability of HACMs. B: Flow cytometry was used to detect cell apoptosis. C-E: RT‒qPCR was used to measure the expression of miR-377-3p, HMOX1, RNA KCNQ1OT1. F: Flow cytometry was used to detect ROS levels. G: MitoSOX Red probe was used to measure mitochondrial ROS levels (scale bar = 100 μm). H-I: Oxidative stress marker kits were used to detect the expression of SOD and MDA. J: Iron analysis kit was utilized to detect changes in Fe²⁺. K: Western blot analysis was used to detecting the expression of CK, LDH, Bax, and Bcl-2. Compared with the NC group, ^* P < 0.05, ^** P < 0.01 and ^*** P < 0.001; compared to with H/R group, ^# P < 0.05, ^## P < 0.01 and ^### P < 0.001; compared with the H/R + miR-377-3p inhibitor group, ^{^}P < 0.05, ^{^^}P < 0.01 and ^{^^^}P < 0.001