Fig. 4

Effect of miR-377-3p on MI/RI (n = 3). A: CCK-8 assay was used to detect cell viability. B: Detection of cell apoptosis by flow cytometry. C-E: Detection of the level of lncRNA-KCNQ1OT1, miR-377-3p, and HMOX1 by RT‒qPCR. F: Flow cytometry analysis of ROS levels. G-H: Oxidative stress marker kits were used to measure SOD and MDA levels. I: MitoSOX Red probe was used to measure mitochondrial ROS levels (scale bar = 100 μm). J: Iron analysis kits were used to measure Fe²⁺ levels. K: CK, LDH, Bax and Bcl-2 expression levels were determined by western bolt. Compared with the NC group, ^** P < 0.01, and ^*** P < 0.001; compared with the H/R group, ^# P < 0.05, ^## P < 0.01 and ^### P < 0.001