Fig. 1

The establishment and detection of I/R damage cell model (n = 3). A-C: Detection of the expression of lncRNA KCNQ1OT1, miR-377-3p and HMOX1 by RT‒qPCR. D: Flow cytometry was used to detect ROS levels. E: MitoSOX Red probe was used to measure mitochondrial ROS levels (scale bar = 100 μm). F-G: Oxidative stress marker kits were utilized to evaluate SOD and MDA levels. H: Iron analysis kits were used to measure Fe²⁺ levels. I: CK and LDH expression levels were determined by western bolt. Compared with the NC group, ^** P < 0.01 and ^***P < 0.001