Fig. 3

Cell surface area and GOT2 protein expression in myocardial cell line H9C2. a WGA staining was used to outline the edges of H9C2 cells (N = 3, n = 3), scale bar: 50 μm. b In situ immunofluorescence detection of GOT2 protein expression in H9C2 cells; red fluorescence represents GOT2 protein, scale bar: 200 μm. c Western blotting was employed to detect the GOT2 protein expression in H9C2 cells (N = 3, n = 3), full-length blots were presented in Supplementary Fig. 3. WGA: wheat germ agglutinin, a specific cardiomyocyte membrane dye; GOT2: glutamic oxaloacetic transaminase 2. N: the number of independent experiments, n: the number of duplicates. Data were expressed as mean ± SD, **p < 0.01