Fig. 2

Ufm1 overexpression activates UFMylation in cardiac tissues of mice. (A, B) Tg and NTg mice were treated with isoproterenol (ISO) or normal saline (NS). qRT-PCR was performed to analyse the transcript levels of Ufm1 (A). The protein expression of Ufm1 was detected using western blotting (B), and the relative expression was calculated by normalizing to β-Tubulin. *P < 0.05 compared between the indicated groups. (C) The heart weight/body weight (HW/BW) ratio in mice as indicated. (D-F) The mRNA levels of Acta1 (D), Anp (E) and BNP (F) were detected using qRT-PCR. Data are shown as mean ± standard deviation *P < 0.05, **P < 0.01, and ****P < 0.0001 between the indicated groups