Fig. 2

Effect of Allicin on cell apoptosis and oxidative stress in cardiomyocytes. H9c2 cells were pretreated with Allicin (0, 25, 50, and 100 µM) for 2 h, followed by incubation with LPS (10 µg/mL) for a further 24 h. (A) Representative TUNEL and DAPI‑stained images. Magnification, x200. (B) Quantitative analysis of TUNEL staining. (C) Cell apoptosis was determined by measurement of caspase-3 activity. The MDA level (D) and SOD activity (E) were measured by respective commercial kits. ***P < 0.001 vs. control group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. LPS group