Fig. 1

Allicin increases the viability of rat cardiomyocytes treated with LPS. H9c2 cells were treated with LPS (10 µg/mL) for 12, 24, and 36 h. (A) Cell viability was assessed by CCK-8 assay. (B) Cell injury was detected by LDH release assay. H9c2 cells were incubated with Allicin (25, 50, and 100 µM) for 24 h, (C) viability and (D) LDH content were measured. H9c2 cells were pretreated with Allicin (0, 25, 50, and 100 µM) for 2 h, followed by incubation with LPS (10 µg/mL) for a further 24 h. (E) CCK-8 assay and (F) LDH release assay was performed. ELISA assay was carried out to measure the concentrations of IL-1β (G), IL-6 (H), and TNF-α (I) in culture media of H9c2 cells. ANOVA was performed to analyze the data. ***P < 0.001 vs. control group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. LPS group