Fig. 1

Changes in cardiac remodelling in rats. a Heart tissue specimens from the rats are shown, and b HW, c HW/BW and d HW/TL were calculated. e Representative images of cardiac cross sections stained with HE were obtained (magnification 200 ×), and the CSA of cardiomyocytes was calculated. Scale bar: 100 µm. f Representative histology of mid-myocardial cross-sections performed with Masson’s trichrome staining was acquired (magnification 200 ×). Scale bar: 100 μm. Quantification of collagen content was analysed. HW: heart weight, HW/BW: heart weight / body weight, HW/TL: heart weight / tibia length, CSA: cross sectional area, CVF: collagen volume fraction. Values are presented as the means ± SDs. *P < 0.05, **P < 0.01, ***P < 0.001 vs CON group by one-way ANOVA; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs DM group by one-way ANOVA