Fig. 3

miR-494-3p affects the proliferation and apoptosis of cardiomyocytes by affecting mitochondrial autophagy

(A) Morphological observation of cardiomyocytes, scale bar = 100 μm. (B) The expression of the autophagy-related proteins LC3 and Beclin 1 was detected by Western blotting. (C) The expression of PINK1 and Parkin was detected by Western blotting. (D) The expression of Mfn1, Mfn2 and OPA1 was detected by Western blotting. (E) The formation of autophagosomes was observed by transmission electron microscopy, scale bar = 5 μm/1 µm/500 nm. (F) Changes in ΔΨm were detected by JC-1 staining. (G) CCK-8 assay of the proliferative activity of cardiomyocytes. (H) Apoptosis of cardiomyocytes was detected by flow cytometry. (I) The expression of the apoptosis-related proteins Bax and Bcl-2 was detected by Western blotting. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001 vs. NC-mimic or NC; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001 vs. NC-inhibitor or miR-494-3p mimic. n = 3, all experiments were repeated three times.