Fig. 2From: Association of circulating MtDNA with CVD in hemodialysis patients and in vitro effect of exogenous MtDNA on cardiac microvascular inflammationHCMECs internalize extracellular MtDNA in the uremic milieu. A PCR analysis of cytochrome B abundance in cytosolic fractions. The internal reference was 18S rDNA. B PCR analysis was performed on TLR9, in which GAPDH was taken as internal reference. Each experiment was performed in triplicate and repeated for three times. *p < 0.05 compared to normal serum group; #p < 0.05 compared to uremic serum group. Data are mean ± SD. NS, normal serum; US, uremic serumBack to article page