Fig. 2

LPS induces ROS production and pyroptosis in H9C2 cells. A Intracellular ROS detected by DCFH-DA (magnification 200 ×). B ROS-associated protein Nox4 detected by western blotting. The cropped blots are shown on the left. The relative protein expression levels compared with the β-actin levels are shown on the right. Full-length blots are presented in Additional file 1: Figure S1A. C Pyroptosis-associated proteins detected by western blotting. The cropped blots are shown on the left. The relative protein expression levels compared with the β-actin levels are shown on the right. Full-length blots are presented in Additional file 1: Figure S1B. D Levels of IL-1β and IL-18 in the culture supernatants. Data are presented as mean ± SD (n = 3). *p < 0.05 compared with the control (CTL) group; **p < 0.01 compared with the CTL group; ***p < 0.001 compared with the CTL group; ns, p > 0.05 compared with the CTL group