Fig. 3
From: HMGB1 promotes Ox-LDL-induced endothelial cell damage by inhibiting PI3K/Akt signaling pathway
Inhibition of HMGB1 expression prevented ox-LDL-induced damage in HUVECs. A Representative protein bands of HMGB1. GAPDH served as a loading control. HMGB1-siRNA: small interfering RNA targeting HMGB1, NC-siRNA: small interfering RNA negative control. Original images of western blots are shown in Additional file 1: Supplementary Fig. 1F, G. B Quantitative analysis of (A) HMGB1 protein bands. C Cell viability in each group detected using the MTT assay. D Cell cytotoxicity detected using a LDH kit. E The percentage of apoptotic cells in each group in F was calculated. F Representative flow cytometry profiles of different treatment groups. ‘+’ was added, ‘–’ was blank. ***p < 0.001