Fig. 2
From: HMGB1 promotes Ox-LDL-induced endothelial cell damage by inhibiting PI3K/Akt signaling pathway
Induction of ox-LDL increases HMGB1 expression. A Representative western blotting images showing HMGB1 protein expression. GAPDH served as an internal control. Original images of western blots are shown in Additional file 1: Supplementary Fig. 1D, E. B The quantification of HMGB1/GAPDH protein ratio. C The HMGB1 mRMA expression level detected using RT-PCR. D Representative fluorescent images of HMGB1 (green) in each group (×400 magnification). The nuclei were stained by DAPI (blue). Scale bar = 100 nm. Each experiment was independently repeated three times and the data were expressed as means ± SEM. **p < 0.01 and ***p < 0.001 versus Control