Fig. 7

Activation of PI3K/Akt signalling pathway by I-Post combined with nicorandil is hindered by PI3K phosphorylation inhibitor wortmannin A Representative sections of TTC/Evans Blue stained heart tissue subjected to 30 min myocardial ischemia followed by 2.5 h of reperfusion and infarct area histogram analysis (n = 5). B Representative western blot showing total expression and the phosphorylation state, Comparison of p-PI3K expression levels in myocardial tissue of rats (n ≥ 3). C Comparison of p-Akt expression levels in myocardial tissue of rats (n ≥ 3). D Comparison of p-GSK3βexpression levels in myocardial tissue of rats (n ≥ 3). E Comparison of p-mTOR expression levels in myocardial tissue of rats(n ≥ 3). F Comparison of p-eNOS expression levels in myocardial tissue of rats (n ≥ 3). Compared with I-Post + Nic + wortmannin: ***P < 0.001. Data represent the mean ± SEM. Data were analysed using T test, one-way ANOVA and Bonferroni multiple comparisons test. I/R, ischaemia‒reperfusion; I-Post + Nic, ischaemia postconditioning, combined with nicorandil; wortmannin, PI3K phosphorylation inhibitor; DM, diabetes mellitus