Fig. 2

Diabetes impairs the cardioprotective effects of I-Post and nicorandil, and the cardioprotective effect of the combination treatment on myocardial I/R injury in diabetic rats. A Representative sections of TTC/Evans Blue stained heart tissue subjected to 30 min myocardial ischemia followed by 2.5 h of reperfusion and infarct area histogram analysis (n = 5). B CK-MB plasma levels in rats treated with I-Post or nicorandil (n = 6). D ROS levels in myocardial tissue in rats treated with I-Post or nicorandil (n = 6). Compared with I/R: ^†††P < 0.001; Compared with DM I/R: ^‡‡‡P < 0.001; Comparison between nondiabetic and diabetic groups applying the same treatment: ***P < 0.001. Data represent the mean ± SEM. Data were analysed using one-way ANOVA and Bonferroni multiple comparisons test. Sham, sham surgery; I/R, ischaemia‒reperfusion; I-Post, ischaemia postconditioning; Nic, nicorandil; DM, diabetes mellitus