Fig. 1

Ox-LDL impaired developments of HUVECs. HUVECs were treated with various doses of ox-LDL (0 μg/mL, 25 μg/mL, 50 μg/mL and 100 μg/mL) for 24 h. A MTT assay was used to detect cell viability in treated HUVECs. B and C Flow cytometry assay was performed to analyze the apoptosis rate in treated HUVECs. D and E Tube formation assay was conducted to determine the tube formation of HUVECs. F and G Western blot assay was applied to detect the protein levels of CyclinD1 and Cleaved-caspase-3 in treated HUVECs. H ELISA assay was used to measure the secretion of inflammatory factors IL-1β and TNF-α in treated HUVECs. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. All tissue and cellular experiments were independently repeated three times. Two-way ANOVA was utilized to analyze the differences in (A, G, and H), while one-way ANOVA was utilized to assess the differences in (B and D)