Fig. 8

a Expression of SIRT1 on days 14 and 28. Immunofluorescence staining of SIRT1 among the groups on days 14 and 28, and representative images are shown here. Scale bar: 50 µm. b Comparison of the AOD of IHC staining of SIRT1 in each group of mice on days 14 and 28 (n = 4–5 mice). c RT-PCR analysis of the relative mRNA expression of SIRT1 in the thrombosed IVC (n = 5 mice). Data are expressed as means ± SD and were analyzed through ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). C: Control group; D: DVT group; ED: exercise and DVT group; IED: SIRT1 inhibitor, exercise DVT group