Elevated Th17 cell proportion, related cytokines and mRNA expression level in patients with hypertension-mediated organ damage: a case control study

Table 2 Primer sequences used in RT-PCR experiments

mRNA	Primer sequences (5' → 3')	Amplicon size (bp)
RORγt	Forward CTCCATCTTTGACTTCTCCCACTCCCTA	247 bp
RORγt	Reverse CACATGCTGGCTACACAGGCTC	247 bp
β-actin	Forward CTGGAACGGTGAAGGTGACA	139 bp
β-actin	Reverse AAGGGACTTCCTGTAACAATGCA	139 bp
STAT3	Forward ACCTGCAGCAATACCATTGAC	122 bp
STAT3	Reverse ACCTGCAGCAATACCATTGAC	122 bp
IL-17	Forward GAATTCCGGCAGGCACAAAC	273 bp
IL-17	Reverse AAGGTGAGGTGGATCGGTTG	273 bp

bp base-pair, RORɣt retinoic acid-related orphan receptor γt, STAT 3 signal transducer and activator of transcription-3, IL interleukin

ISSN: 1471-2261