Fig. 4

Silencing of G9a promotes BDNF expression by repressing H3K9me2 modification of the BDNF promoter. A Interaction of G9a with downregulated genes visualized using PPI network. B Detection of BDNF expression in sera of HF patients (n = 40) and healthy subjects (n = 40) by RT-qPCR. C Pearson’s analysis of the correlation between the expression of G9a and BDNF. D Detection of BDNF expression in myocardial tissues of rats (n = 6) by RT-qPCR. E Analysis of BDNF expression in cells by Western blot (Uncropped protein blots are provided in the Additional file 1). F The expression of H3K9me2 in cells by Western blot (Uncropped protein blots are provided in the Additional file 1). G ChIP-seq data of H3K9me2 enrichment peaks on BDNF promoter (n = 17). H Detection of BDNF promoter enriched by Anti-G9a or Anti-H3K9me2 by ChIP-qPCR. Data are expressed as mean ± SD. Each experiment was repeated at least three times unless otherwise indicated. A comparison of data from 2 groups was performed by an unpaired t test, while 3 or more groups were compared by one-way or two-way ANOVA and Tukey's post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001