Table 4 The immunocytochemical evaluation of HSP27 expression in: (A) H9C2 cells, (B) HCM cells
Incubation time [h] | Custodiol HTK | Plegisol | Control cells |
|---|---|---|---|
(A) | |||
0.5 | < 5%* −/+ | < 5%* + | 45% ++ |
1 | 18%* + | 20%* + | |
2 | 20–12% + | 40% + | |
4 | 25%* + | 70%* +/++ | |
(B) | |||
0.5 | No reaction | <5% + | 45% ++ |
1 | No reaction | 30% +/++ | |
2 | No reaction | 50%* +/++ | |
4 | < 5%* +/++ | no reaction | |