Fig. 5

MVP suppressed cardiomyocyte apoptosis via activating AKT signaling pathway in DiCM mice. A, B Western blots and statistical results of MVP, p-AKT, and AKT in heart tissue 5 weeks after AAV9 injection. (n = 6 or 7). C, D Western blots and statistical results of Bcl-2, Bax, c-Caspase 3 and Caspase 3 in heart tissue 5 weeks after AAV9 injection. (n = 6 or 7). E The bar graph shows the TUNEL-positive cell ratio (%) as a cellular apoptotic index. The TUNEL-stained cells (green) were counted and normalized by the DAPI-stained cell (blue). F Representative fluorescence microscopic TUNEL images. (n = 6). Values represent the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001