Fig. 4

MVP protected DOX-induced cardiac fibrosis mitochondrial injury in mice. A Representative images of HE staining of heart are shown 5 weeks after AAV9 injection. B, E Representative images and the analysis of Masson’s trichrome staining of cardiomyocyte, and the images of perivascular area, are shown 5 weeks after AAV9 injection. C, F Representative immunohistochemistry images and the analysis of cardiac sections stained with MVP 5 weeks after AAV9 injection. D Representative TEM images in mice heart. Mitochondrial can be identified by higher electro density (dark areas). Bottom images show enlarged detail of area in top images that contain mitochondria. G Increase mitochondrial fitness in cardiomyocyte in the DOX + MVP group compared with DOX + Vec as defined from TEM images and quantified as percent of cristae area per mitochondrial. Each dot represents one mitochondria. Data are shown as means from 30 mitochondria and 6 mice per group. N = 6, values represent the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001