Fig. 3
From: A20 (TNFAIP3) alleviates viral myocarditis through ADAR1/miR-1a-3p-dependent regulation
A20 suppresses inflammation and apoptosis of CVB3-infected cardiomyocytes. Notes qRT-PCR (A) and western blotting (B) detected the expression of A20 in primary neonatal rat cardiomyocytes and H9c2 cells. CVB3-infected primary neonatal rat cardiomyocytes and H9c2 cells were transfected with LV-sh-A20, LV-sh-NC, LV-A20 or LV-NC. qRT-PCR (C) and western blotting (D) detected the expression of A20 in the cells; ELISA measured the levels of IL-6 (E), IL-18 (F) and TNF-α (G) in the supernatant; H flow cytometry assessed the cell apoptosis. *P < 0.05; **P < 0.01; ***P < 0.001; CVB3 Coxsackie virus B3