Fig. 4

The mRNA levels of Col I and Col III were decreased, while the mRNA levels of Sirt 1 were increased by RSV in rats with DCM. The mRNA levels of Col I, Col III and Sirt1 were measured by qRT-PCR. β-actin was amplified as an internal control. The fold change (2^−ΔΔCT) was calculated to evaluate the mRNA levels. Values were presented as mean ± SD. a, b The mRNA levels of Col I, Col III in the heart on the 21st, 56th, 90th day after the first immunization. c–e The mRNA levels of Col I, Col III and Sirt1 in the heart on the 90th day after the first immunization. Col I, collagen type I; Col III, collagen type III; Sirt1, Silence information regulator 1; DCM, dilated cardiomyopathy; RSV-L, low dose of RSV (10 mg/kg/d); RSV-H, high dose of RSV (50 mg/kg/d); ***, P < 0.0001; *, P < 0.05