Fig. 5

WB and RT-qPCR verification of the two novel candidate key TFs, MAFF and MAFG. a, b RT-qPCR validation results. c–f WB validation results. The change trends in MAFF and MAFG in the RT-qPCR and WB analysis results were consistent with the change trends in the TFRE results, so MAFF and MAFG were validated as two novel candidate key TFs in AS. * indicates 0.01 < P < 0.05, ** indicates 0.001 < P < 0.01, and *** indicates P < 0.001