Table 1 Specific primers in RT-qPCR assay
Gene | Direction (5′–3′) | Sequence |
|---|---|---|
circ_0004104 | Forward | 5′-AGACCTGTGACCTGGACAATG-3′ |
Reverse | 5′-GTGCACTTTGTGGCAAAGAA-3′ | |
SPARC | Forward | 5′-GGTATCTGTGGGAGCTAATC-3′ |
Reverse | 5′-CTGGTGGGGTCCTGGCACAC-3′ | |
miR-328-3p | Forward | 5′-CCTCTCTGCCCTTCCG-3′ |
Reverse | 5′-GAACATGTCTGCGTATCTC-3′ | |
TRIM14 | Forward | 5′-GAGGTCGGAGCTTGTCGAG-3′ |
Reverse | 5′-TTCTTGGCTGAGTTTCTGCAC-3′ | |
U6 | Forward | 5′-CTCGCTTCGGCAGCACA-3′ |
Reverse | 5′-AACGCTTCACGAATTTGCGT-3′ | |
GAPDH | Forward | 5′-AAGAAGGTGGTGAAGCAGGC-3′ |
Reverse | 5′-GTCAAAGGTGGAGGAGTGGG-3′ |