Fig. 5

Circ_0004104 silencing attenuates ox-LDL-induced dysfunction in HUVECs partly through up-regulating miR-328-3p. a The interference efficiency of anti-miR-328-3p in HUVECs was tested by RT-qPCR. This experiment was performed for three times with three technical repetitions. b Cell viability was analyzed by MTT assay. This experiment was performed for three times with six technical repetitions. c Flow cytometry was carried out to analyze cell apoptosis rate in different groups. This experiment was performed for three times with three technical repetitions. d The angiogenic ability was analyzed using capillary-like network formation assay in vitro. This experiment was performed for three times with three technical repetitions. e, f Western blot assay was applied to analyze the levels of Cleaved-casp3 and VEGFA in transfected HUVECs. This experiment was performed for three times. g ELISA was utilized to assess the levels of TNF-α and IL-1β in the supernatant of HUVECs. This experiment was performed for three times with three technical repetitions. h The levels of SOD and MDA were detected using their matching commercial kits. This experiment was performed for three times with three technical repetitions. *P < 0.05, **P < 0.01, ***P < 0.001. Student’s t-test was utilized to analyze the differences in (a), whereas one-way ANOVA was utilized to assess the differences in (b–h)