Fig. 4

BM-MSCs treatment decreased the cardiac apoptotic cell numbers after MIRI. Representative images (200×) of myocardial TUNEL staining of MIRI mice treated with IgG, IgG + BM-MSCs, PC61, PC61 + BM-MSCs, splenectomy, and splenectomy + BM-MSCs are shown (a). The hearts were collected 72 h after surgically-induced MIRI. Arrows indicate the apoptotic cells. The scalebars shown in the lower right corner of each panel represent 50 μm. From the images, the number of apoptotic cells were counted and expressed in mean value ± standard deviation of each indicated group (n = 5) (b). BM-MSCs, bone marrow-derived mesenchymal stem cells; MIRI, myocardial ischemia–reperfusion injury