Analysis for interaction between interleukin-35 genes polymorphisms and risk factors on susceptibility to coronary heart disease in the Chinese Han population

Table 1 Description of 4 SNPs and the assays or primers designed for genotyping

SNPs	Chromosome	Functional Consequence	Major/minor alleles	Genotyping assays or primers
EBI3	19:4229916	Intron variant	G > C	GAATTTGAGTCACACTCATTCCTTT[C/G]
rs428253	19:4229916	Intron variant	G > C	GTTTCTTTTTGGTTTTGTTTTTTGA
EBI3	19:4236999	Missense variant, coding sequence variant	G > A	TGTGCGGCCCCGAGCCAGGTACTAC[A/G]
rs4740	19:4236999	Missense variant, coding sequence variant	G > A	TCCAAGTGGCGGCTCAGGACCTCAC
IL-12A rs2243115	3:159988493	Upstream transcript variant, intron variant	T > G	Forward: 5′-AGAAAAGACCTGTGAACAAAACGACT-3′
IL-12A rs2243115	3:159988493	Upstream transcript variant, intron variant	T > G	Reverse: 5′-AGATGGCTCACTAGATGCCAGG-3′
IL-12A rs568408	3:159995680	3 prime UTR variant, intron variant	G > A	Forward: 5′-GAAGGATGGGACYATTACATCCATAT-3′
IL-12A rs568408	3:159995680	3 prime UTR variant, intron variant	G > A	Reverse: 5′-CAGGATGGATATTTTCCCTTCT-3′

ISSN: 1471-2261