Fig. 2
Shear stress enhanced the CAD-derived EPCs proliferation, adhesion, and migration capacities in vitro. a-c: CAD-derived EPCs were exposed to shear stress treatment for 6, 12, and 24 h, separately, at 0, 5, 15, and 30 dyn/cm2. a: Quantitative analysis and typical images for EPCs migration (**P < 0.01 vs. Static CAD-EPCs; n = 3 in each group). b: Quantitative analysis and typical images for EPCs adhesion (*p < 0.05/**p < 0.01vs. Static CAD-EPCs; n = 3 in each group). c: Quantitative analysis for EPCs proliferation (*p < 0.05/**p < 0.01vs. Static CAD-EPCs; n = 3 in each group). d: Quantitative analysis for the mRNA levels of CXCR7 and ERK in EPCs treated with or without 12 h of shear stress at 15 dyn/cm2 determined through Real-time PCR (n = 3 in each group). e: Quantitative analysis and typical images for the protein levels of CXCR7 and p-ERK/ERK in EPCs treated with or without 12 h of shear stress at 15 dyn/cm2 measured by Western Blotting (*p < 0.05/**p < 0.01vs. Static CAD-EPCs; n = 3 in each group)