Fig. 6

L-T4 treatment increased the expression of BMP4/Smad4 protein in the offspring of SCH pregnant rats. a-b Expression level of BMP4 and Smad4 mRNA in the offspring of SCH pregnant rats; c-d Representative Western blot images and quantitative analysis of BMP4 and Smad4 intensity in each group; e-f) Representative immunohistochemical images of BMP4 and Smad4 expression and quantitative analysis of BMP4- and Smad4- positive cells in each group (Scale bar = 50 μm). All experiments were repeated at least three times. Data were expressed as the mean ± SEM (n = 5 per group). ^*p < 0.05 vs Sham group; ^#p < 0.05 vs SCH group