Fig. 3

Effect of rolipram on the inflammatory response in the hearts of endotoxic mice. Endotoxic mice were established by intraperitoneal injection of 15 mg/kg LPS for 6 h. In the rolipram group, 10 mg/kg rolipram was injected intraperitoneally 1 h before LPS injection. The hearts of the mice were dissociated, and the mRNA and protein levels of proinflammatory molecules (TNF-ɑ, IL-6 and IL-1β) and DUSP1 were measured by qRT-PCR and western blotting assays. a, Relative fold of changes to control of DUSP1 mRNA expression in heart tissues. b, Representative image of western blots of DUSP1 expression (original blot was shown in supplement Fig. S1D). c-e, Relative fold of changes to control of TNF-ɑ, IL-6 and IL-1β mRNA expression in heart tissues. f-h, The concentration of TNF-ɑ, IL-6 and IL-1β in heart tissues. * represents P < 0.05, n = 5