Fig. 5

MORT is likely an upstream inhibitor of miR-93 in AC16 cells. AC16 cells were transfected with either MORT expression vector or miR-93 mimic to analyse the interaction between MORT and miR-93. MORT and miR-93 overexpression as well as MORT silencing was achieved in cells of AC16 cell line at 36 h after transfection (a). MORT overexpression led to downregulated and MORT siRNA silencing led to upregulated miR-93 expression (b), while miR-93 overexpression did not significantly affect MORT expression (c) (*, p < 0.05)