Fig. 6
Fibrosis. Picro-Sirius Red staining of cardiac tissue from the AF group (n = 5) and the control group (n = 3). A + B: Representative images of left atrial appendage (LAA, panel A) and right atrial appendage (RAA, panel B) sections, respectively. Fibrosis is stained red. C: Quantification of Picro-Sirius Red positive areas for full sections of the three regions: LAA, RAA and right ventricle (RV). Collagen was significantly increased in the LAA in the AF group (black) compared to the control group (grey). D + E: Representative images showing areas of interstitial fibrosis for LAA (D) and RAA (E). F: Quantification of Picro-Sirius Red positive areas showed a significant increase in interstitial fibrosis for the AF group in the LAA compared to the control group. Sections for RV not shown. *p < 0.05. Scale bar = 500 μm in (A and B) and 100 μm in (D and E)