Table 1 Primers in qPCR
Gene | Forward Primer | Reverse Primer |
|---|---|---|
NEAT1 | 5′ GCCAGTGTGAGTCCTAGCATTG 3′ | 5′ ACTTCCTCCTCCTAAGCCTCTG 3′ |
miR-193a | 5′ ACACTCCAGCTGGGACTGGGACTTTGTAGGCCA 3′ | 5′ TGTCGTGGAGTCGGCAATTC 3’ |
miR-182 | 5′ ACACTCCAGCTGGGTTTGGCAATGGTAGAACT 3’ | 5′ TGTCGTGGAGTCGGCAATTC 3’ |
miR-141 | 5′ ACACTCCAGCTGGGTAACACTGTCTGGTAAAG 3’ | 5′ TGTCGTGGAGTCGGCAATTC 3’ |
U6 | 5′ CGCTTCGGCAGCACATATACTA 3’ | 5′ ATGGAACGCTTCACGAATTTGC 3’ |
GAPDH | 5′ GAGTCCACTGGCGTCTTCAC 3’ | 5′ ATCTTGAGGCTGTTGTCATACTTCT 3’ |